Relationship between microbial community, operational factors and ammonia inhibition resilience in anaerobic digesters at low and moderate ammonia background concentrations

Article

Lu, Y., Liaquat, R., Astals, S., Jensen, P. D., Batstone, D. J. and Tait, S.. 2018. "Relationship between microbial community, operational factors and ammonia inhibition resilience in anaerobic digesters at low and moderate ammonia background concentrations." New Biotechnology. 44, pp. 23-30. https://doi.org/10.1016/j.nbt.2018.02.013
Article Title

Relationship between microbial community, operational factors and ammonia inhibition resilience in anaerobic digesters at low and moderate ammonia background concentrations

ERA Journal ID3336
Article CategoryArticle
AuthorsLu, Y. (Author), Liaquat, R. (Author), Astals, S. (Author), Jensen, P. D. (Author), Batstone, D. J. (Author) and Tait, S. (Author)
Journal TitleNew Biotechnology
Journal Citation44, pp. 23-30
Number of Pages8
Year2018
Place of PublicationNetherlands
ISSN1389-0344
1871-6784
Digital Object Identifier (DOI)https://doi.org/10.1016/j.nbt.2018.02.013
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S1871678417306957?via%3Dihub
Abstract

The relationship between anaerobic digestion operational conditions and (i) microbial community, (ii) acetoclastic methanogenic activity and (iii) free ammonia (NH3) inhibition resilience was investigated. Thirteen inocula were obtained from full and pilot scale digesters fed with different substrates, digester configurations, operating temperatures and NH3 concentrations (0.1–241 mgN·L−1). Substrate type and temperature were the primary factors influencing microbial community composition. Methanogenic activity ranged from 0.04 to 0.14 gCOD-CH4·g−1VS·day−1, and was significantly correlated with archaeal relative abundance and archaeal community PC2. The variability of NH3 resilience among inocula was moderate, with inhibition threshold values (KI50) ranging between 32 and 175 mgNH3-N·L−1. No microbial or operational factors correlated with NH3 resilience. However, the slopes of inhibition threshold curves were influenced by some environmental factors, namely substrate type, digester temperature and NH3 concentration. Overall, these results indicate that low and moderate background NH3 concentrations is not a key determinant of microbial community nor NH3 resilience.

Keywordsanaerobic digestion, inhibition, toxicity, nitrogen, microbial community, acetoclastic methanogenesis
ANZSRC Field of Research 2020401102. Environmentally sustainable engineering
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Byline AffiliationsUniversity of Queensland
Quaid-i-Azam University, Pakistan
Institution of OriginUniversity of Southern Queensland
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