The density of a protein crystal

Article

White, E. T., Tan, Wei Hong, Ang, Jing Mei, Tait, S. and Litster, J. D.. 2007. "The density of a protein crystal." Powder Technology. 179 (1-2), pp. 55-58. https://doi.org/10.1016/j.powtec.2006.11.014
Article Title

The density of a protein crystal

ERA Journal ID3918
Article CategoryArticle
AuthorsWhite, E. T. (Author), Tan, Wei Hong (Author), Ang, Jing Mei (Author), Tait, S. (Author) and Litster, J. D. (Author)
Journal TitlePowder Technology
Journal Citation179 (1-2), pp. 55-58
Number of Pages4
Year2007
Place of PublicationNetherlands
ISSN0032-5910
1873-328X
Digital Object Identifier (DOI)https://doi.org/10.1016/j.powtec.2006.11.014
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0032591006005018
Abstract

The density of a crystal of protein can vary by large amounts (e.g. 25%) depending on the solution from which it is recovered. This is because protein molecules (MW 10 to 1000 kDa) are so big that when they build into a crystal lattice, the interstices between molecules are large enough to include water and ions. In some cases, up to half the volume of a protein crystal can be the included liquid whose density depends on its composition. As the liquor density is changed, the overall crystal density will change. This paper investigates the changes in the density of crystals of the proteins lysozyme and ovalbumin as the crystals are part-dried. Crystal density was measured using a helium pycnometer. Measurements of the equilibrium moisture content of lysozyme crystals in contact with humid air are also reported.

KeywordsProtein; Crystallization; Crystal density
ANZSRC Field of Research 2020400406. Powder and particle technology
Byline AffiliationsUniversity of Queensland
Institution of OriginUniversity of Southern Queensland
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