Ammonia stress on a resilient mesophilic anaerobic inoculum: methane production, microbial community, and putative metabolic pathways
Article
Article Title | Ammonia stress on a resilient mesophilic anaerobic inoculum: methane production, microbial community, and putative metabolic pathways |
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ERA Journal ID | 3454 |
Article Category | Article |
Authors | Buhlmann, Christopher H. (Author), Mickan, Bede S. (Author), Jenkins, Sasha N. (Author), Tait, Stephan (Author), Kahandawala, Tharanga K. A. (Author) and Bahri, Parisa A. (Author) |
Journal Title | Bioresource Technology |
Journal Citation | 275, pp. 70-77 |
Number of Pages | 8 |
Year | 2018 |
Publisher | Elsevier |
Place of Publication | Netherlands |
ISSN | 0960-8524 |
1873-2976 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biortech.2018.12.012 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0960852418316560 |
Abstract | Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400–10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ± 0.0003 gCOD.gVS−1inoculum.day−1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of ‘gene hits’ for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities. |
Keywords | anaerobic digestion; inhibition; methanogenesis; metabolic pathways; PICRUSt |
ANZSRC Field of Research 2020 | 401102. Environmentally sustainable engineering |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Murdoch University |
University of Western Australia | |
University of Queensland | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q5177/ammonia-stress-on-a-resilient-mesophilic-anaerobic-inoculum-methane-production-microbial-community-and-putative-metabolic-pathways
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