HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa
Article
Article Title | HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa |
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ERA Journal ID | 2939 |
Article Category | Article |
Authors | Windus, Louisa C.E. (Author), Jones, Adele M. (Author), Downes, Sharon (Author), Walsh, Tom (Author), Kinkema, Mark (Author) and Knight, Kristen (Author) |
Journal Title | Journal of Invertebrate Pathology |
Journal Citation | 183, pp. 1-8 |
Article Number | 107598 |
Number of Pages | 8 |
Year | 2021 |
Publisher | Elsevier |
Place of Publication | United States |
ISSN | 0022-2011 |
1096-0805 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jip.2021.107598 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0022201121000653 |
Abstract | Genetically engineered crops expressing insecticidal toxins from Bacillus thuringiensis (Bt) have improved the management of targeted lepidopteran pests and reduced the use of insecticide sprays. These benefits explain an increasing adoption of Bt crops worldwide, intensifying the selection pressure on target species and the risk of resistance. Nucleopolyhedroviruses (NPVs) are effective bioinsecticides against numerous important lepidopteran pests. If Bt-resistant insects are shown to be susceptible to NPVs then these bioinsecticides could be a valuable component of Insecticide Resistance Management (IRM) strategies for Bt crops. We assessed the effectiveness of a Helicoverpa nucleopolyhedrovirus (HearNPV) against several different Bt-resistant strains. Utilising a droplet feeding bioassay we confirmed susceptibility to HearNPV in Helicoverpa punctigera and Helicoverpa armigera larvae resistant to the Bt toxins Cry1Ac, Cry2Ab, and Vip3A. Dual resistant H. punctigera, (Cry1Ac/Cry2Ab, and Cry2Ab/Vip3A) and dual resistant H. armigera (Cry2Ab/Vip3A) were also susceptible to HearNPV. Regardless of their specific resistance profile, Bt-resistant larvae displayed statistically similar lethal concentration (LC50) and lethal time (LT50) responses to HearNPV when compared to Bt-sensitive control insects. These results indicate that Bt-resistant H. armigera and H. punctigera are not cross-resistant to HearNPV. Consequently, the use of HearNPV against these pests may be a valuable tool to an IRM strategy for controlling Bt-resistant populations. |
Keywords | Bt resistance; Cross-resistance; HearNPV; Helicoverpa armigera; Helicoverpa punctigera; Nucleopolyhedrovirus |
ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
Byline Affiliations | AgBiTech, Australia |
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia | |
AgBiTech, United States | |
Bayer Crop Science, Australia | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q6vyw/hearnpv-susceptibility-in-helicoverpa-armigera-and-helicoverpa-punctigera-strains-resistant-to-bt-toxins-cry1ac-cry2ab-and-vip3aa
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