HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa

Article

Windus, Louisa C.E., Jones, Adele M., Downes, Sharon, Walsh, Tom, Kinkema, Mark and Knight, Kristen. 2021. "HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa." Journal of Invertebrate Pathology. 183, pp. 1-8. https://doi.org/10.1016/j.jip.2021.107598
Article Title

HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa

ERA Journal ID2939
Article CategoryArticle
AuthorsWindus, Louisa C.E. (Author), Jones, Adele M. (Author), Downes, Sharon (Author), Walsh, Tom (Author), Kinkema, Mark (Author) and Knight, Kristen (Author)
Journal TitleJournal of Invertebrate Pathology
Journal Citation183, pp. 1-8
Article Number107598
Number of Pages8
Year2021
PublisherElsevier
Place of PublicationUnited States
ISSN0022-2011
1096-0805
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jip.2021.107598
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0022201121000653
Abstract

Genetically engineered crops expressing insecticidal toxins from Bacillus thuringiensis (Bt) have improved the management of targeted lepidopteran pests and reduced the use of insecticide sprays. These benefits explain an increasing adoption of Bt crops worldwide, intensifying the selection pressure on target species and the risk of resistance. Nucleopolyhedroviruses (NPVs) are effective bioinsecticides against numerous important lepidopteran pests. If Bt-resistant insects are shown to be susceptible to NPVs then these bioinsecticides could be a valuable component of Insecticide Resistance Management (IRM) strategies for Bt crops. We assessed the effectiveness of a Helicoverpa nucleopolyhedrovirus (HearNPV) against several different Bt-resistant strains. Utilising a droplet feeding bioassay we confirmed susceptibility to HearNPV in Helicoverpa punctigera and Helicoverpa armigera larvae resistant to the Bt toxins Cry1Ac, Cry2Ab, and Vip3A. Dual resistant H. punctigera, (Cry1Ac/Cry2Ab, and Cry2Ab/Vip3A) and dual resistant H. armigera (Cry2Ab/Vip3A) were also susceptible to HearNPV. Regardless of their specific resistance profile, Bt-resistant larvae displayed statistically similar lethal concentration (LC50) and lethal time (LT50) responses to HearNPV when compared to Bt-sensitive control insects. These results indicate that Bt-resistant H. armigera and H. punctigera are not cross-resistant to HearNPV. Consequently, the use of HearNPV against these pests may be a valuable tool to an IRM strategy for controlling Bt-resistant populations.

KeywordsBt resistance; Cross-resistance; HearNPV; Helicoverpa armigera; Helicoverpa punctigera; Nucleopolyhedrovirus
ANZSRC Field of Research 2020300409. Crop and pasture protection (incl. pests, diseases and weeds)
Byline AffiliationsAgBiTech, Australia
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
AgBiTech, United States
Bayer Crop Science, Australia
Institution of OriginUniversity of Southern Queensland
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