Regulation of the chemokine receptors CXCR7 and CXCR4 in 3-D culture models of prostate cancer

Poster


Kiss, Debra L., Windus, Louisa C. E. and Avery, Vickey M.. 2012. "Regulation of the chemokine receptors CXCR7 and CXCR4 in 3-D culture models of prostate cancer." 2012 AACR Special Conference on Advances in Prostate Cancer Research. Orlando, United States of America 06 - 09 Feb 2012 United States. https://doi.org/10.1158/1538-7445
Paper/Presentation Title

Regulation of the chemokine receptors CXCR7 and CXCR4 in 3-D culture models of prostate cancer

Presentation TypePoster
AuthorsKiss, Debra L. (Author), Windus, Louisa C. E. (Author) and Avery, Vickey M. (Author)
Journal or Proceedings TitleProceedings of the 2012 AACR Special Conference on Advances in Prostate Cancer Research
Journal Citation72
Year2012
Place of PublicationUnited States
Digital Object Identifier (DOI)https://doi.org/10.1158/1538-7445
Web Address (URL) of Paperhttps://cancerres.aacrjournals.org/content/72/4_Supplement/C50.short
Conference/Event2012 AACR Special Conference on Advances in Prostate Cancer Research
Event Details
2012 AACR Special Conference on Advances in Prostate Cancer Research
Event Date
06 to end of 09 Feb 2012
Event Location
Orlando, United States of America
Abstract

CXCR7 was recently identified as the second member of the chemokine receptor family to bind stromal derived factor-1α (SDF-1α), a chemokine which is known to influence the establishment of cancer metastasis. Whilst expression of CXCR7 is highly restricted in non-malignant cells, it is widely expressed in many different tumor cell lines. In prostate cancer, a disease known to be highly regulated by the other SDF-1α -binding receptor CXCR4, CXCR7 has been found to regulate cell growth and invasion. In vivo prostate tumor biopsies show a pattern where CXCR7 expression increases with invasive grade, as previously reported for CXCR4. However, there is limited knowledge on the role of CXCR7 and its function in prostate cancer.

In this study, we aim to more thoroughly characterize CXCR7 function across prostate cancer cell lines, in particular its regulation of cell growth and behavior. We will also assess its expression and function in response to its ligands and inhibitors, alongside CXCR4, in order to assess how these receptors are regulated in relation to each other. For this we employ western blotting expression studies, immunocytochemical visualization, and metabolic-based proliferation assays.

Further, we will study the regulation of both CXCR7 and CXCR4 in 3D culture models of prostate cancer cell lines. Our preliminary data from 2D culture suggests that less invasive prostate cancer cell lines express higher levels of CXCR7 than more invasive cell lines, contrary to reports in vivo where CXCR7 expression was seen to increase with invasive grade. We have chosen to assess these aspects in 3D cultures of prostate cancer cell lines to determine whether culturing in 3D permits a phenotype more reflective of what has been reported for CXCR7 expression in prostate cancer in vivo.

Further elucidation of CXCR7 function with respect to CXCR4 will shed light on how these receptors contribute to regulation of the metastatic process in prostate cancer – a process known to be heavily regulated by CXCR4. As CXCR4 has been established as a therapeutic target in prostate cancer, a more detailed knowledge of the CXCR7 receptor with which it shares a partial redundancy may indicate whether combinatorial therapies may be more effective in combating prostate cancer progression.

Keywordsprostate cancer
ANZSRC Field of Research 2020310110. Receptors and membrane biology
Public Notes

Abstract #C50.

Byline AffiliationsGriffith University
Institution of OriginUniversity of Southern Queensland
Permalink -

https://research.usq.edu.au/item/q5z41/regulation-of-the-chemokine-receptors-cxcr7-and-cxcr4-in-3-d-culture-models-of-prostate-cancer

  • 102
    total views
  • 45
    total downloads
  • 2
    views this month
  • 1
    downloads this month

Export as

Related outputs

Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases
Windus, Louisa C. E., Matigian, Nicholas and Avery, Vicky M.. 2023. "Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases." Biology. 12 (6). https://doi.org/10.3390/biology12060861
Fundamentals of Anatomy and Physiology
Chruscik, Anna, Kauter, Kate, Windus, Louisa, Whiteside, Eliza and Dooley, Leanne. 2021. Fundamentals of Anatomy and Physiology . Australia. University of Southern Queensland.
HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa
Windus, Louisa C.E., Jones, Adele M., Downes, Sharon, Walsh, Tom, Kinkema, Mark and Knight, Kristen. 2021. "HearNPV susceptibility in Helicoverpa armigera and Helicoverpa punctigera strains resistant to Bt toxins Cry1Ac, Cry2Ab, and Vip3Aa." Journal of Invertebrate Pathology. 183, pp. 1-8. https://doi.org/10.1016/j.jip.2021.107598
The impact of an anatomy and physiology open textbook on student satisfaction and engagement in a regional Australian university
Chruscik, Anna, Kauter, Kate, Whiteside, Anna and Windus, Louisa. 2022. "The impact of an anatomy and physiology open textbook on student satisfaction and engagement in a regional Australian university." 39th International Conference on Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education (ASCILITE 2022). Sydney, Australia 04 - 07 Dec 2022 Australia. Australasian Society for Computers in Learning in Tertiary Education (ASCILITE). https://doi.org/10.14742/APUBS.2022.183
How to do science: A guide to researching human physiology
Lexis, Louise, Julien, Brianna, Bliss, Edward, Derrington, Kate, Dooley, Leanne, Kauter, Kate, McGregor, Rowena, Myer, Sarah, Salmeron, Raquel, Suraweera, Samanthi, Whiteside, Eliza and Windus, Louisa. 2022. How to do science: A guide to researching human physiology. Australia. University of Southern Queensland.
Academic achievement and student satisfaction: moving anatomy and physiology teaching online
Chruscik, Anna, Windus, Louisa, Wang, Jianxiong, Kauter, Kate and Whiteside, Eliza. 2020. "Academic achievement and student satisfaction: moving anatomy and physiology teaching online." 2020 Australian Conference on Science and Mathematics Education (formerly UniServe Science Conference). Online 30 Sep - 02 Oct 2020 Australia.
Solving the supply of Resveratrol Tetramers from Papua New Guinean rainforest Anisoptera species (Dipterocarpaceae) that inhibit bacterial type III secretion systems
Davis, Rohan A., Beattie, Karren D., Xu, Min, Yang, Xinzhou, Yin, Sheng, Holla, Harish, Healy, Peter C., Sykes, Melissa, Shelper, Todd, Avery, Vicky M., Elofsson, Mikael, Sundin, Charlotta and Quinn, Ronald. 2014. "Solving the supply of Resveratrol Tetramers from Papua New Guinean rainforest Anisoptera species (Dipterocarpaceae) that inhibit bacterial type III secretion systems." Journal of Natural Products. 77 (12), pp. 2633-2640. https://doi.org/10.1021/np500433z
Chemokine receptor expression on integrin-mediated stellate projections of prostate cancer cells in 3D culture
Kiss, Debra, Windus, Louisa C. E. and Avery, Vicky. 2013. "Chemokine receptor expression on integrin-mediated stellate projections of prostate cancer cells in 3D culture." Cytokine. 64 (1), pp. 122-130. https://doi.org/10.1016/j.cyto.2013.07.012
Bone-stromal cells up-regulate tumourigenic markers in a tumour-stromal 3D model of prostate cancer
Windus, Louisa C. E., Glover, Tristan T. and Avery, V.. 2013. "Bone-stromal cells up-regulate tumourigenic markers in a tumour-stromal 3D model of prostate cancer." Molecular Cancer. 12, pp. 1-19. https://doi.org/10.1186/1476-4598-12-112
PCaAnalyser: A 2D-Image Analysis Based Module for Effective Determination of Prostate Cancer Progression in 3D Culture
Hoque, Md Tamjidul, Windus, Louisa C. E., Lovitt, Carrie J. and Avery, Vicky. 2013. "PCaAnalyser: A 2D-Image Analysis Based Module for Effective Determination of Prostate Cancer Progression in 3D Culture." PLoS One. 8 (11), pp. 1-13. https://doi.org/10.1371/journal.pone.0079865
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer
Windus, Louisa C. E., Kiss, Debra L., Glover, Tristan T. and Avery, Vicky M.. 2012. "In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer." Experimental Cell Research. 318 (19), pp. 2507-2519. https://doi.org/10.1016/j.yexcr.2012.07.013
Correction of aberrant axon growth in the developing mouse olfactory bulb
Chan, C., Ekberg, J., Lineburg, K., Scott, S., Chehrehasa, F., Windus, L., Claxton, C., Mackay-Sim, A., Key, B. and St John, J.. 2011. "Correction of aberrant axon growth in the developing mouse olfactory bulb." Molecular and Cellular Neuroscience. 46 (1), pp. 282-295. https://doi.org/10.1016/j.mcn.2010.09.012
OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro
Ekberg, J., Amaya, D., Chehrehasa, F., Lineburg, K., Claxton, C., Windus, Louisa C. E., Key, B., Mackay-Sim, A. and St John, J.. 2011. "OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro." Journal of Neuroscience Methods. 196 (1), pp. 88-98. https://doi.org/10.1016/j.jneumeth.2011.01.008
Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth
Windus, Louisa C. E., Chehrehasa, Fatemah, Lineburg, Katie, Claxton, Christina, Mackay-Sim, Alan, Key, Brian and St John, James. 2011. "Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth." Cellular and Molecular Life Sciences. 68 (19), pp. 3233-3247. https://doi.org/10.1007/s00018-011-0630-9
Copper, Nickel, and Zinc Cyclam−Amino Acid and Cyclam−Peptide Complexes May Be Synthesized with 'Click' Chemistry and Are Noncytotoxic
Yu, Mingfeng, Price, Jason, Jensen, Paul D., Lovitt, Carrie J., Shelper, Todd, Duffy, Sandra, Windus, Louisa C., Avery, Vicky, Rutledge, Peter and Todd, Matthew. 2011. "Copper, Nickel, and Zinc Cyclam−Amino Acid and Cyclam−Peptide Complexes May Be Synthesized with 'Click' Chemistry and Are Noncytotoxic." Inorganic Chemistry: including bioinorganic chemistry. 50, pp. 12823-12835. https://doi.org/10.1021/ic2020012
Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions
Windus, Louisa C. E., Lineburg, Katie, Scott, Susan, Claxton, Christina, Mackay-Sim, Alan, Key, Brian and St John, James. 2010. "Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions." Cellular and Molecular Life Sciences. 67 (10), pp. 1735-1750. https://doi.org/10.1007/s00018-010-0280-3
Olfactory glia enhance neonatal axon regeneration
Chehrehasa, Fatemah, Windus, Louisa C. E., Ekberg, Jenny, Scott, Susan, Amaya, Daniel, Mackay-Sim, Alan and St John, James. 2010. "Olfactory glia enhance neonatal axon regeneration." Molecular and Cellular Neuroscience. 45 (3), pp. 277-288. https://doi.org/10.1016/j.mcn.2010.07.002
Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia
Windus, Louisa C. E., Claxton, C., Allen, C., Key, B. and St John, J.. 2007. "Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia." Glia. 55 (15), pp. 1708-1719. https://doi.org/10.1002/glia.20586
Expression profiling in monozygotic twins discordant for bipolar disorder reveals dysregulation of the WNT signalling pathway
Matigan, N., Windus, L., Smith, H., Filippich, C., Pantelis, C., McGrath, J., Mowry, B. and Hayward, N.. 2007. "Expression profiling in monozygotic twins discordant for bipolar disorder reveals dysregulation of the WNT signalling pathway." Molecular Psychiatry. 12 (9), pp. 815-825. https://doi.org/10.1038/sj.mp.4001998
Evidence of altered prefrontal–thalamic circuitry in schizophrenia: An optimized diffusion MRI study
Rose, S., Chalk, J., Janke, A., Strudwick, M., Windus, Louisa C., Hannah, D., McGrath, J., Pantelis, C., Wood, S. and Mowry, B.. 2006. "Evidence of altered prefrontal–thalamic circuitry in schizophrenia: An optimized diffusion MRI study." NeuroImage. 32 (1), pp. 16-22. https://doi.org/10.1016/j.neuroimage.2006.03.003
Separate and interacting effects within the catechol-O-methyltransferase (COMT) are associated with schizophrenia
Handoko, HY, Nyholt, DR, Hayward, NK, Nertney, DA, Hannah, DE, Windus, LC, McCormack, CM, Smith, HJ, Flippich, C, James, MR and Mowry, BJ. 2004. "Separate and interacting effects within the catechol-O-methyltransferase (COMT) are associated with schizophrenia." Molecular Psychiatry. 10 (6), pp. 589-597. https://doi.org/10.1038/sj.mp.4001606