Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions

Article


Windus, Louisa C. E., Lineburg, Katie, Scott, Susan, Claxton, Christina, Mackay-Sim, Alan, Key, Brian and St John, James. 2010. "Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions." Cellular and Molecular Life Sciences. 67 (10), pp. 1735-1750. https://doi.org/10.1007/s00018-010-0280-3
Article Title

Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions

ERA Journal ID2192
Article CategoryArticle
AuthorsWindus, Louisa C. E. (Author), Lineburg, Katie (Author), Scott, Susan (Author), Claxton, Christina (Author), Mackay-Sim, Alan (Author), Key, Brian (Author) and St John, James (Author)
Journal TitleCellular and Molecular Life Sciences
Journal Citation67 (10), pp. 1735-1750
Number of Pages16
Year2010
Place of PublicationSwitzerland
ISSN1420-682X
1420-9071
Digital Object Identifier (DOI)https://doi.org/10.1007/s00018-010-0280-3
Web Address (URL)https://link.springer.com/article/10.1007%2Fs00018-010-0280-3
Abstract

The growth and guidance of primary olfactory axons are partly attributed to the presence of olfactory ensheathing cells (OECs). However, little is understood about the differences between the subpopulations of OECs and what regulates their interactions. We used OEC-axon assays and determined that axons respond differently to peripheral and central OECs. We then further purified OECs from anatomically distinct regions of the olfactory bulb. Cell behaviour assays revealed that OECs from the olfactory bulb were a functionally heterogeneous population with distinct differences which is consistent with their proposed roles in vivo. We found that the heterogeneity was regulated by motile lamellipodial waves along the shaft of the OECs and that inhibition of lamellipodial wave activity via Mek1 abolished the ability of the cells to distinguish between each other. These results demonstrate that OECs from the olfactory bulb are a heterogeneous population that use lamellipodial waves to regulate cell–cell recognition.

KeywordsOlfactory bulb; Axon; Regeneration; Glia; Mek1
ANZSRC Field of Research 2020310105. Cellular interactions (incl. adhesion, matrix, cell wall)
310102. Cell development, proliferation and death
Byline AffiliationsGriffith University
Institution of OriginUniversity of Southern Queensland
Funding source
NHMRC
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