Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth

Article

Windus, Louisa C. E., Chehrehasa, Fatemah, Lineburg, Katie, Claxton, Christina, Mackay-Sim, Alan, Key, Brian and St John, James. 2011. "Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth." Cellular and Molecular Life Sciences. 68 (19), pp. 3233-3247. https://doi.org/10.1007/s00018-011-0630-9
Article Title

Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth

ERA Journal ID2192
Article CategoryArticle
AuthorsWindus, Louisa C. E. (Author), Chehrehasa, Fatemah (Author), Lineburg, Katie (Author), Claxton, Christina (Author), Mackay-Sim, Alan (Author), Key, Brian (Author) and St John, James (Author)
Journal TitleCellular and Molecular Life Sciences
Journal Citation68 (19), pp. 3233-3247
Number of Pages15
Year2011
Place of PublicationSwitzerland
ISSN1420-682X
1420-9071
Digital Object Identifier (DOI)https://doi.org/10.1007/s00018-011-0630-9
Web Address (URL)https://link.springer.com/article/10.1007%2Fs00018-011-0630-9
Abstract

Axons of primary olfactory neurons are intimately associated with olfactory ensheathing cells (OECs) from the olfactory epithelium until the final targeting of axons within the olfactory bulb. However, little is understood about the nature and role of interactions between OECs and axons during development of the olfactory nerve pathway. We have used high resolution time-lapse microscopy to examine the growth and interactions of olfactory axons and OECs in vitro. Transgenic mice expressing fluorescent reporters in primary olfactory axons (OMP-ZsGreen) and ensheathing cells (S100ß-DsRed) enabled us to selectively analyse these cell types in explants of olfactory epithelium. We reveal here that rather than providing only a permissive substrate for axon growth, OECs play an active role in modulating the growth of pioneer olfactory axons. We show that the interactions between OECs and axons were dependent on lamellipodial waves on the shaft of OEC processes. The motility of OECs was mediated by GDNF, which stimulated cell migration and increased the apparent motility of the axons, whereas loss of OECs via laser ablation of the cells inhibited olfactory axon outgrowth. These results demonstrate that the migration of OECs strongly regulates the motility of axons and that stimulation of OEC motility enhances axon extension and growth cone activity.

KeywordsNeuron; OEC; GDNF; Lamellipodia; Glia; ZsGreen
ANZSRC Field of Research 2020310102. Cell development, proliferation and death
310110. Receptors and membrane biology
Byline AffiliationsGriffith University
Institution of OriginUniversity of Southern Queensland
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