Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases

Article

Windus, Louisa C. E., Matigian, Nicholas and Avery, Vicky M.. 2023. "Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases." Biology. 12 (6). https://doi.org/10.3390/biology12060861
Article Title

Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases

ERA Journal ID210193
Article CategoryArticle
AuthorsWindus, Louisa C. E., Matigian, Nicholas and Avery, Vicky M.
Journal TitleBiology
Journal Citation12 (6)
Article Number861
Number of Pages22
Year2023
PublisherMDPI AG
Place of PublicationSwitzerland
ISSN2079-7737
Digital Object Identifier (DOI)https://doi.org/10.3390/biology12060861
Web Address (URL)https://www.mdpi.com/2079-7737/12/6/861
AbstractWhen prostate cancer cells spread to other parts of the body (metastasise), they frequently migrate to the bone. Here they encounter a microenvironment that differs greatly from that in the primary tumour (prostate), namely interactions with bone-specific cells, including fibroblasts. To better understand the contribution of these fibroblasts to tumour progression, it is the aim of the current paper to propagate a biologically relevant fibroblast population that mimics the cellular and molecular profile of fibroblasts as found in the metastatic bone environment in the body. Using three-dimensional (3D) cell culture models, we were able to transform normal fibroblast cells into fibroblasts that express modified protein and gene profiles similar to those found in fibroblasts in the metastatic bone environment in the body. The exploitation of these engineered 3D models could help further unravel the novel biology regulating metastatic growth and the role fibroblasts play in the colonisation process. Elucidation of these processes has the potential to aid in the development of therapies not currently available for late-stage cancers.
Keywordsprostate cancer; 3D cultures; EMT/MET; tumour–stromal microenvironment; reactive bone stromal fibroblasts
ANZSRC Field of Research 2020321101. Cancer cell biology
Byline AffiliationsGriffith University
University of Queensland
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