Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI
Article
Article Title | Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI |
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ERA Journal ID | 1429 |
Article Category | Article |
Authors | Liu, Tongle (Author), Rao, Yuan (Author), Zhou, Wenfeng (Author), Zhuang, Wei (Author), Ge, Lei (Author), Lin, Rijia (Author), Zhang, Wenxia (Author), Wu, Jinglan (Author), Zhu, Chenjie (Author) and Ying, Hanjie (Author) |
Journal Title | Colloids and Surfaces B: Biointerfaces |
Journal Citation | 205, pp. 1-10 |
Article Number | 111888 |
Number of Pages | 10 |
Year | 2021 |
Publisher | Elsevier |
Place of Publication | Netherlands |
ISSN | 0927-7765 |
1873-4367 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.colsurfb.2021.111888 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0927776521003325 |
Abstract | Adenylate cyclase (AC) can efficiently catalyze the conversion of adenosine triphosphate (ATP) to cyclic adenosine-3′, 5′-monophosphate (cAMP). However, AC directly immobilized on substrate is not desirable due to enzyme inactivation. Herein, bio-inspired adhesive of polydopamine and polyethyleneimine (PDA/PEI) was used as flexible chains to graft on graphene oxide (GO), and the AC was directionally immobilized through affinity between metal ions and his-tags of AC. The properties of modified GO and the activity of immobilized AC were studied in detail. PDA/PEI layers have been proved to improve the amino density of GO surface for affinity groups decoration and adjust the interaction between AC and support. And modified GO by this novel method contributes to subsequent grafting and immobilization of AC by affinity. AC immobilized on modified GO exhibited high activity recovery with about 90 % of free AC, while enzyme immobilized on unmodified GO has been inactivated. This study offers a versatile approach for support modification and enzyme oriented immobilization. PDA/PEI functionalized GO can be used as a promising carrier to immobilize other his-tagged enzymes. |
Keywords | Adenylate cyclase; Affinity; Graphene oxide; Polydopamine; Polyethyleneimine |
ANZSRC Field of Research 2020 | 340201. Bioinorganic chemistry |
400408. Reaction engineering (excl. nuclear reactions) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Nanjing Tech University, China |
Centre for Future Materials | |
University of Queensland | |
Zhengzhou University, China | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q77q0/improved-adenylate-cyclase-activity-via-affinity-immobilization-onto-co-modified-go-with-bio-inspired-adhesive-and-pei
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