Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes
Article
Article Title | Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: matching between molecular dimensions and hierarchical pore sizes |
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ERA Journal ID | 9642 |
Article Category | Article |
Authors | Zhuang, Wei (Author), Huang, Jinsha (Author), Liu, Xiaojing (Author), Ge, Lei (Author), Niu, Huanqing (Author), Wang, Zhenfu (Author), Wu, Jinglan (Author), Yang, Pengpeng (Author), Chen, Yong (Author) and Ying, Hanjie (Author) |
Journal Title | Food Chemistry |
Journal Citation | 275, pp. 197-205 |
Year | 2019 |
Place of Publication | Netherlands |
ISSN | 0308-8146 |
1873-7072 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.foodchem.2018.09.077 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0308814618316492?via%3Dihub |
Abstract | To achieve efficient one-step production of gluconic acid, cascade reactions of glucose oxidase (GOD) and catalase (CAT) have been advocated in the biocatalysis system. In this work, the methodology of co-immobilization of GOD and CAT was investigated in details for obtaining improved enzyme loading and activity. The maximum adsorption capability of GOD and CAT was 24.18 and 14.33 mg.g(-1), respectively. The matching between dimensions of enzymes and hierarchical pore sizes of carriers are critical to the success of immobilization process. The simultaneous self-assembly on glutaraldehyde cross-linked mesoporous carriers exhibited favorable properties in comparison with sequential immobilization of GOD and CAT. The conversion of glucose under adequate air by co-localized GOD&CAT sustained the activity more than 90% after repeated utilization in the production of sodium gluconate and gluconic acid, suggesting that the co-immobilized GOD&CAT could be a promising catalyst for gluconate and gluconic acid production in some chemical and food industries. |
Keywords | pore structure; gluconic acid; adsorption capacity; self-assembly approach; multi-enzyme immobilization; ACID production; nuclease p-1; enzyme immobilization; adenosine-deaminase; mesoporous tio2; performance; resin; glutaraldehyde; catalysis; cascade |
ANZSRC Field of Research 2020 | 340399. Macromolecular and materials chemistry not elsewhere classified |
400408. Reaction engineering (excl. nuclear reactions) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Nanjing Tech University, China |
Centre for Future Materials | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q5038/co-localization-of-glucose-oxidase-and-catalase-enabled-by-a-self-assembly-approach-matching-between-molecular-dimensions-and-hierarchical-pore-sizes
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