Reversible Adsorption and Detachment of Saccharomyces cerevisiae on Thermoresponsive Poly(N-isopropylacrylamide)-Grafted Fibers for Continuous Immobilized Fermentation
Article
Article Title | Reversible Adsorption and Detachment of Saccharomyces cerevisiae on Thermoresponsive Poly(N-isopropylacrylamide)-Grafted Fibers for Continuous Immobilized Fermentation |
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ERA Journal ID | 1479 |
Article Category | Article |
Authors | Sha, Yu, Zhao, Chenchen, Zhuang, Wei, Chen, Jiale, Liu, Dong, Chen, Yong Chen, Ge, Lei, Wu, Jinglan, Zhu, Chenjie, Liu, Jinle and Ying, Hanjie |
Journal Title | Langmuir: the ACS journal of surfaces and colloids |
Journal Citation | 38 (50), pp. 15827-15838 |
Number of Pages | 12 |
Year | 2022 |
Publisher | American Chemical Society |
Place of Publication | United States |
ISSN | 0743-7463 |
1520-5827 | |
Digital Object Identifier (DOI) | https://doi.org/10.1021/acs.langmuir.2c02758 |
Web Address (URL) | https://pubs.acs.org/doi/epdf/10.1021/acs.langmuir.2c02758 |
Abstract | Biofilm-mediated continuous fermentation with cells immobilized has gained much attention in recent years. In this study, thermoresponsive poly(N-isopropylacrylamide)-grafted cotton fibers (PNIPAM-CF) were prepared via an improved surface-initiated atom transfer radical polymerization. The modification process imparted switchable wettability to the surface while maintaining the thermal stability and biocompatibility of the CF. During the ethanol transformation, the rapid, reversible cell adsorption and detachment of Saccharomyces cerevisiae were performed through the modulation of wettability, displaying the enhancement of immobilized biomass and immobilization efficiency from 2.20 g/L and 59.43% to 2.81 g/L and 93.32%, respectively. Moreover, the biofilm adsorption matched well with the Freundlich model, indicating that multilayer adhesion was the main mode of biofilm formation. Based on the accumulation of the biofilm, the fabrication and utilization of PNIPAM-CF improved the efficiency of continuous immobilized fermentation, making the ethanol production reach 26.34 g/L in the sixth batch of fermentation. Meanwhile, wettability regulation further enhanced the reusability of the carrier. Therefore, the findings of this study revealed that the application of smart materials in cell immobilization systems had broad prospects for achieving sustainable and continuous catalysis. |
ANZSRC Field of Research 2020 | 340301. Inorganic materials (incl. nanomaterials) |
400408. Reaction engineering (excl. nuclear reactions) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Nanjing Tech University, China |
Centre for Future Materials | |
Zhengzhou University, China |
https://research.usq.edu.au/item/z02qy/reversible-adsorption-and-detachment-of-saccharomyces-cerevisiae-on-thermoresponsive-poly-n-isopropylacrylamide-grafted-fibers-for-continuous-immobilized-fermentation
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