Central role of manganese in regulation of stress responses, physiology, and metabolism in streptococcus pneumoniae
Article
Article Title | Central role of manganese in regulation of stress responses, physiology, and metabolism in streptococcus pneumoniae |
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ERA Journal ID | 2483 |
Article Category | Article |
Authors | Ogunniyi, Abiodun D. (Author), Mahdi, Layla K. (Author), Jennings, Michael P. (Author), McEwan, Alastair G. (Author), McDevitt, Christopher A. (Author), Van der Hoek, Mark B. (Author), Bagley, Christopher J. (Author), Hoffmann, Peter (Author), Gould, Katherine A. (Author) and Paton, James C. (Author) |
Journal Title | Journal of Bacteriology |
Journal Citation | 192 (17), pp. 4489-4497 |
Number of Pages | 9 |
Year | 2010 |
Publisher | American Society for Microbiology |
Place of Publication | United States |
ISSN | 0021-9193 |
1067-8832 | |
1098-5530 | |
Digital Object Identifier (DOI) | https://doi.org/10.1128/JB.00064-10 |
Web Address (URL) | https://journals.asm.org/doi/10.1128/JB.00064-10 |
Abstract | The importance of Mn(2+) for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn(2+) is required are yet to be fully elucidated. Here, we analyzed the effect of Mn(2+) limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn(2+) transporter, pneumococcal surface antigen A (PsaA), with its isogenic wild-type counterpart. We provide clear evidence for the Mn(2+)-dependent regulation of the expression of oxidative-stress-response enzymes SpxB and Mn(2+)-SodA and virulence-associated genes pcpA and prtA. We also demonstrate the upregulation of at least one oxidative- and nitrosative-stress-response gene cluster, comprising adhC, nmlR, and czcD, in response to Mn(2+) stress. A significant increase in 6-phosphogluconate dehydrogenase activity in the psaA mutant grown under Mn(2+) replete conditions and upregulation of an oligopeptide ABC permease (AppDCBA) were also observed. Together, the results of transcriptomic and proteomic analyses provided evidence for Mn(2+) having a central role in activating or stimulating enzymes involved in central carbon and general metabolism. Our results also highlight the importance of high-affinity Mn(2+) transport by PsaA in pneumococcal competence, physiology, and metabolism and elucidate mechanisms underlying the response to Mn(2+) stress. |
Keywords | ABC permease; bacterial antigen; gene product; manganese; oligopeptide; permease; phosphogluconate dehydrogenase; pneumococcal surface antigen A; protein soda; protein SpxB; proteome; transcriptome; unclassified drug; adhesion; bacterial protein; lipoprotein; manganese; proteome; PsaA protein; Streptococcus |
ANZSRC Field of Research 2020 | 310701. Bacteriology |
320701. Medical bacteriology | |
310109. Proteomics and intermolecular interactions (excl. medical proteomics) | |
Byline Affiliations | University of Adelaide |
Griffith University | |
University of Queensland | |
University of London, United Kingdom | |
Institution of Origin | University of Southern Queensland |
Funding source | NHMRC Grant ID 284214 |
https://research.usq.edu.au/item/q35x4/central-role-of-manganese-in-regulation-of-stress-responses-physiology-and-metabolism-in-streptococcus-pneumoniae
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