The variable region of the pneumococcal pathogenicity island 1 is responsible for the unusually high virulence of a serotype 1 isolate

Article


Harvey, Richard M., Trappetti, Claudia, Mahdi, Layla K., Wang, Hui, McAllister, Lauren J., Scalvini, Alexandra, Paton, Adrienne W. and Paton, James C.. 2016. "The variable region of the pneumococcal pathogenicity island 1 is responsible for the unusually high virulence of a serotype 1 isolate." Infection and Immunity. 84 (3), pp. 822-832. https://doi.org/10.1128/IAI.01454-15
Article Title

The variable region of the pneumococcal pathogenicity island 1 is responsible for the unusually high virulence of a serotype 1 isolate

ERA Journal ID16226
Article CategoryArticle
AuthorsHarvey, Richard M. (Author), Trappetti, Claudia (Author), Mahdi, Layla K. (Author), Wang, Hui (Author), McAllister, Lauren J. (Author), Scalvini, Alexandra (Author), Paton, Adrienne W. (Author) and Paton, James C. (Author)
Journal TitleInfection and Immunity
Journal Citation84 (3), pp. 822-832
Number of Pages11
Year2016
Place of PublicationUnited States
ISSN0019-9567
1098-5522
Digital Object Identifier (DOI)https://doi.org/10.1128/IAI.01454-15
Web Address (URL)https://journals.asm.org/doi/10.1128/IAI.01454-15
Abstract

Streptococcus pneumoniae is the leading infectious cause of death in children in the world. However, the mechanisms that drive the progression from asymptomatic colonization to disease are poorly understood. Two virulence-associated genomic accessory regions (ARs) were deleted in a highly virulent serotype 1 clinical isolate (strain 4496) and examined for their contribution to pathogenesis. Deletion of a prophage encoding a platelet-binding protein (PblB) resulted in reduced adherence, biofilm formation, reduced initial infection within the lungs, and a reduction in the number of circulating platelets in infected mice. However, the region's overall contribution to the survival of mice was not significant. In contrast, deletion of the variable region of pneumococcal pathogenicity island 1 (vPPI1) was also responsible for a reduction in adherence and biofilm formation but also reduced survival and invasion of the pleural cavity, blood, and lungs. While the 4496ΔPPI1 strain induced higher expression of the genes encoding interleukin-10 (IL-10) and CD11b in the lungs of challenged mice than the wild-type strain, very few other genes exhibited altered expression. Moreover, while the level of IL-10 protein was increased in the lungs of 4496ΔPPI1 mutant-infected mice compared to strain 4496-infected mice, the levels of gamma interferon (IFN-γ), CXCL10, CCL2, and CCL4 were not different in the two groups. However, the 4496ΔPPI1 mutant was found to be more susceptible than the wild type to phagocytic killing by a macrophage-like cell line. Therefore, our data suggest that vPPI1 may be a major contributing factor to the heightened virulence of certain serotype 1 strains, possibly by influencing resistance to phagocytic killing.

KeywordsStreptococcus pneumoniae; mutations; vPPI1; mouse model
ANZSRC Field of Research 2020310701. Bacteriology
320603. Medical molecular engineering of nucleic acids and proteins
Public Notes

File reproduced in accordance with the copyright policy of the publisher/author.

Byline AffiliationsUniversity of Adelaide
Institution of OriginUniversity of Southern Queensland
Funding source
NHMRC
Grant ID
565526
Funding source
NHMRC
Grant ID
1071659
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