Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease

Article


Mahdi, Layla K., Ogunniyi, Abiodun D., LeMessurier, Kim S. and Paton, James C.. 2008. "Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease ." Infection and Immunity. 76 (2), pp. 646-657. https://doi.org/10.1128/IAI.01161-07
Article Title

Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease

ERA Journal ID16226
Article CategoryArticle
AuthorsMahdi, Layla K. (Author), Ogunniyi, Abiodun D. (Author), LeMessurier, Kim S. (Author) and Paton, James C. (Author)
Journal TitleInfection and Immunity
Journal Citation76 (2), pp. 646-657
Number of Pages12
Year2008
Place of PublicationUnited States
ISSN0019-9567
1098-5522
Digital Object Identifier (DOI)https://doi.org/10.1128/IAI.01161-07
Web Address (URL)http://iai.asm.org/content/76/2/646
Abstract

Pneumococcal disease continues to account for significant morbidity and mortality worldwide. For the development of novel prophylactic and therapeutic strategies against the disease spectrum, a complete understanding of pneumococcal behavior in vivo is necessary. We evaluated the expression patterns of the proven and putative virulence factor genes adcR, cbpA, cbpD, cbpG, cpsA, nanA, pcpA, piaA, ply, psaA, pspA, and spxB after intranasal infection of CD1 mice with serotype 2, 4, and 6A pneumococci by real-time reverse transcription- PCR. Simultaneous gene expression patterns of selected host immunomodulatory molecules, CCL2, CCL5, CD54, CXCL2, interleukin-6, and tomor necrosis factor alpha, were also investigated. We show that pneumococcal virulence genes are differentially expressed in vivo, with some genes demonstrating niche- and serotype-specific differential expression. The in vivo expression patterns could not be attributed to in vitro differences in expression of the genes in transparent and opaque variants of the three strains. The host molecules were significantly upregulated, especially in the lungs, blood, and brains of mice. The pneumococcal-gene expression patterns support their ascribed roles in pathogenesis, providing insight into which protein combinations might be more appropriate as vaccine antigens against invasive disease. This is the first simultaneous comparison of bacterial- and host gene expression in the same animal during pathogenesis. The strategy provides a platform for prospective evaluation of interaction kinetics between invading pneumococci and human patients in culture-positive cases and should be feasible in other infection models.

Keywordsanimals; bacterial proteins; blood; brain; colony count,microbial; cytokines; female; gene expression profiling; lung; mice; nasal cavity; pneumococcal infections; reverse transcriptase polymerase chain reaction; virulence factors
ANZSRC Field of Research 2020310701. Bacteriology
310704. Microbial genetics
320701. Medical bacteriology
Public Notes

File reproduced in accordance with the copyright policy of the publisher/author.

Byline AffiliationsUniversity of Adelaide
Institution of OriginUniversity of Southern Queensland
Funding source
NHMRC
Grant ID
284214
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