Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease
Article
Article Title | Pneumococcal virulence gene expression and host cytokine profiles during pathogenesis of invasive disease |
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ERA Journal ID | 16226 |
Article Category | Article |
Authors | Mahdi, Layla K. (Author), Ogunniyi, Abiodun D. (Author), LeMessurier, Kim S. (Author) and Paton, James C. (Author) |
Journal Title | Infection and Immunity |
Journal Citation | 76 (2), pp. 646-657 |
Number of Pages | 12 |
Year | 2008 |
Place of Publication | United States |
ISSN | 0019-9567 |
1098-5522 | |
Digital Object Identifier (DOI) | https://doi.org/10.1128/IAI.01161-07 |
Web Address (URL) | http://iai.asm.org/content/76/2/646 |
Abstract | Pneumococcal disease continues to account for significant morbidity and mortality worldwide. For the development of novel prophylactic and therapeutic strategies against the disease spectrum, a complete understanding of pneumococcal behavior in vivo is necessary. We evaluated the expression patterns of the proven and putative virulence factor genes adcR, cbpA, cbpD, cbpG, cpsA, nanA, pcpA, piaA, ply, psaA, pspA, and spxB after intranasal infection of CD1 mice with serotype 2, 4, and 6A pneumococci by real-time reverse transcription- PCR. Simultaneous gene expression patterns of selected host immunomodulatory molecules, CCL2, CCL5, CD54, CXCL2, interleukin-6, and tomor necrosis factor alpha, were also investigated. We show that pneumococcal virulence genes are differentially expressed in vivo, with some genes demonstrating niche- and serotype-specific differential expression. The in vivo expression patterns could not be attributed to in vitro differences in expression of the genes in transparent and opaque variants of the three strains. The host molecules were significantly upregulated, especially in the lungs, blood, and brains of mice. The pneumococcal-gene expression patterns support their ascribed roles in pathogenesis, providing insight into which protein combinations might be more appropriate as vaccine antigens against invasive disease. This is the first simultaneous comparison of bacterial- and host gene expression in the same animal during pathogenesis. The strategy provides a platform for prospective evaluation of interaction kinetics between invading pneumococci and human patients in culture-positive cases and should be feasible in other infection models. |
Keywords | animals; bacterial proteins; blood; brain; colony count,microbial; cytokines; female; gene expression profiling; lung; mice; nasal cavity; pneumococcal infections; reverse transcriptase polymerase chain reaction; virulence factors |
ANZSRC Field of Research 2020 | 310701. Bacteriology |
310704. Microbial genetics | |
320701. Medical bacteriology | |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Byline Affiliations | University of Adelaide |
Institution of Origin | University of Southern Queensland |
Funding source | NHMRC Grant ID 284214 |
https://research.usq.edu.au/item/q35xx/pneumococcal-virulence-gene-expression-and-host-cytokine-profiles-during-pathogenesis-of-invasive-disease
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