Application of RAPD- PCR to differentiate among Aeromonas spp
Article
Article Title | Application of RAPD- PCR to differentiate among Aeromonas spp |
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Article Category | Article |
Authors | Jaladet, M. S. J. (Author), M., Layla Kh. (Author) and Zaineb, M. N. (Author) |
Journal Title | Journal of Al-Nahrain University |
Journal Citation | 7, pp. 48-56 |
Number of Pages | 9 |
Year | 2004 |
Place of Publication | Baghdad, Iraq |
Abstract | Eleven Aeromonas isolates were examined for their biotypes, antibiogram and RAPD fingerprints.The biochemical analysis revealed three species (Aeromonas caviae, Aeromonas hydrophila, Aeromonas sobria). While antibiogram analysis gave rise to six patterns of resistance. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to differentiate the genus Aeromonas at the genomospecies level. Of the (24) primers evaluated, (11) produced profiles, which contain multiple, bands capable of differentiating the genomospecies. In most cases, each isolate gave a unique fingerprint, illustrating genetic heterogeneity at the genomospecies level. However, some homogeneity in fragment sizes was seen among isolates within a genomospecies, which was not apparent in strains from different species, RAPD-PCR with primers OPN-7 and OPN-13 revealed eight patterns. Whereas primer OPN-9 yielded only three patterns. These results showed that RAPD-PCR has the potential to differentiate Aeromonas. RAPD analysis can be used in epidemiological studies for the identification and characterization of Aeromonas. |
ANZSRC Field of Research 2020 | 310199. Biochemistry and cell biology not elsewhere classified |
Byline Affiliations | Duhook University, Iraq |
Al-Nahrain University, Iraq | |
Al-Kadimiya Teaching Hospital, Iraq | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q35y8/application-of-rapd-pcr-to-differentiate-among-aeromonas-spp
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