The pneumococcal alpha-glycerophosphate oxidase enhances nasopharyngeal colonization through binding to host glycoconjugates
Article
Article Title | The pneumococcal alpha-glycerophosphate oxidase enhances |
---|---|
ERA Journal ID | 210392 |
Article Category | Article |
Authors | Mahdi, Layla K. (Author), Higgins, Melanie A. (Author), Day, Christopher J. (Author), Tiralongo, Joe (Author), Hartley-Tassell, Lauren E. (Author), Jennings, Michael P. (Author), Gordon, David L. (Author), Paton, Adrienne W. (Author), Paton, James C. (Author) and Ogunniyi, Abiodun D. (Author) |
Journal Title | EBioMedicine |
Journal Citation | 18, pp. 236-243 |
Number of Pages | 8 |
Year | 2017 |
Place of Publication | Netherlands |
ISSN | 2352-3964 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.ebiom.2017.03.002 |
Web Address (URL) | http://www.sciencedirect.com/science/article/pii/S2352396417300890 |
Abstract | Streptococcus pneumoniae (the pneumococcus) is a major human pathogen, causing a broad spectrum of diseases including otitis media, pneumonia, bacteraemia and meningitis. Here we examined the role of a potential pneumococcal meningitis vaccine antigen, alpha-glycerophosphate oxidase (SpGlpO), in nasopharyngeal colonization. We found that serotype 4 and serotype 6A strains deficient in SpGlpO have significantly reduced capacity to colonize the nasopharynx of mice, and were significantly defective in adherence to human nasopharyngeal carcinoma cells in vitro. We also demonstrate that intranasal immunization with recombinant SpGlpO significantly protects mice against subsequent nasal colonization by wild type serotype 4 and serotype 6A strains. Furthermore, we show that SpGlpO binds strongly to lacto/neolacto/ganglio host glycan structures containing the GlcNAcβ1-3Galβ disaccharide, suggesting that SpGlpO enhances colonization of the nasopharynx through its binding to host glycoconjugates. We propose that SpGlpO is a promising vaccine candidate against pneumococcal carriage, and warrants inclusion in a multi-component protein vaccine formulation that can provide robust, serotype-independent protection against all forms of pneumococcal disease. |
Keywords | bacterial pathogens, Streptococcus pneumoniae; protein vaccines; pneumococcal disease; colonization; adherence; host glycoconjugates; alpha-glycerophosphate oxidase; immunization |
ANZSRC Field of Research 2020 | 310701. Bacteriology |
Byline Affiliations | University of Adelaide |
Griffith University | |
Flinders University | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q3y6q/the-pneumococcal-alpha-glycerophosphate-oxidase-enhances-nasopharyngeal-colonization-through-binding-to-host-glycoconjugates
Download files
923
total views100
total downloads0
views this month0
downloads this month