The pneumococcal alpha-glycerophosphate oxidase enhances nasopharyngeal colonization through binding to host glycoconjugates

Article


Mahdi, Layla K., Higgins, Melanie A., Day, Christopher J., Tiralongo, Joe, Hartley-Tassell, Lauren E., Jennings, Michael P., Gordon, David L., Paton, Adrienne W., Paton, James C. and Ogunniyi, Abiodun D.. 2017. "The pneumococcal alpha-glycerophosphate oxidase enhances nasopharyngeal colonization through binding to host glycoconjugates." EBioMedicine. 18, pp. 236-243. https://doi.org/10.1016/j.ebiom.2017.03.002
Article Title

The pneumococcal alpha-glycerophosphate oxidase enhances
nasopharyngeal colonization through binding to host
glycoconjugates

ERA Journal ID210392
Article CategoryArticle
AuthorsMahdi, Layla K. (Author), Higgins, Melanie A. (Author), Day, Christopher J. (Author), Tiralongo, Joe (Author), Hartley-Tassell, Lauren E. (Author), Jennings, Michael P. (Author), Gordon, David L. (Author), Paton, Adrienne W. (Author), Paton, James C. (Author) and Ogunniyi, Abiodun D. (Author)
Journal TitleEBioMedicine
Journal Citation18, pp. 236-243
Number of Pages8
Year2017
Place of PublicationNetherlands
ISSN2352-3964
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ebiom.2017.03.002
Web Address (URL)http://www.sciencedirect.com/science/article/pii/S2352396417300890
Abstract

Streptococcus pneumoniae (the pneumococcus) is a major human pathogen, causing a broad spectrum of diseases including otitis media, pneumonia, bacteraemia and meningitis. Here we examined the role of a potential pneumococcal meningitis vaccine antigen, alpha-glycerophosphate oxidase (SpGlpO), in nasopharyngeal colonization. We found that serotype 4 and serotype 6A strains deficient in SpGlpO have significantly reduced capacity to colonize the nasopharynx of mice, and were significantly defective in adherence to human nasopharyngeal carcinoma cells in vitro. We also demonstrate that intranasal immunization with recombinant SpGlpO significantly protects mice against subsequent nasal colonization by wild type serotype 4 and serotype 6A strains. Furthermore, we show that SpGlpO binds strongly to lacto/neolacto/ganglio host glycan structures containing the GlcNAcβ1-3Galβ disaccharide, suggesting that SpGlpO enhances colonization of the nasopharynx through its binding to host glycoconjugates. We propose that SpGlpO is a promising vaccine candidate against pneumococcal carriage, and warrants inclusion in a multi-component protein vaccine formulation that can provide robust, serotype-independent protection against all forms of pneumococcal disease.

Keywordsbacterial pathogens, Streptococcus pneumoniae; protein vaccines; pneumococcal disease; colonization; adherence; host glycoconjugates; alpha-glycerophosphate oxidase; immunization
ANZSRC Field of Research 2020310701. Bacteriology
Byline AffiliationsUniversity of Adelaide
Griffith University
Flinders University
Institution of OriginUniversity of Southern Queensland
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