Preparation of C-reactive protein latex reagent of human serum

Article

Alshalchi, Sahar A., Mahdi, Layla K. and Sabar, Muna. 2006. "Preparation of C-reactive protein latex reagent of human serum ." Iraqi Journal of Biotechnology. 5 (1), pp. 112-122.

Article Title	Preparation of C-reactive protein latex reagent of human serum
Article Category	Article
Authors	Alshalchi, Sahar A. (Author), Mahdi, Layla K. (Author) and Sabar, Muna (Author)
Journal Title	Iraqi Journal of Biotechnology
Journal Citation	5 (1), pp. 112-122
Number of Pages	11
Year	2006
Place of Publication	Bagdad
Web Address (URL)	http://en.jige.uobaghdad.edu.iq/uploads/2006.a.1/9.pdf
Abstract	C-reactive protein (CRP) is an acute phase reactant the concentration of which increases significantly following tissue injury or inflammation. It is present in the albumin fraction of the serum of patients and is antigenic upon injection into rabbits. We report here the development of a local latex reagent coated with rabbit anti-CRP. For this purpose CRP was isolated from pooled sera of patients and injected into rabbits. CRP rabbit antiserum was purified by using ammonium sulfate precipitation. Followed by passing on ion exchange chromatography (Diethylaminoethyl 'DEAE' cellulose). Then, latex particles were coated with purified antisera. The prepared CRP latex reagent agglutination was observed upon the addition of patients' sera and compared with that of normal sera. In addition, it was evaluated against a standard CRP latex reagent. The prepared reagent gave positive results (100%) with patients' sera and the positive control of the standard kit. While, the negative control of the standard kit and 30% of the normal sera gave negative results.
Keywords	CRP, latex agglutination
ANZSRC Field of Research 2020	320203. Clinical microbiology
	310701. Bacteriology
	320211. Infectious diseases
Byline Affiliations	University of Baghdad, Iraq
	Al-Nahrain University, Iraq
	No affiliation
Institution of Origin	University of Southern Queensland

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