The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype

Article


Thomas, Patrick B., Jeffrey, Penny, Gahete, Manuel D., Whiteside, Eliza, Walpole, Carina, Maugham, Michelle, Jovanovic, Lidija, Gunter, Jennifer, Williams, Elizabeth, Nelson, Colleen, Herington, Adrian, Luque, Raul M., Veedu, Rakesh, Chopin, Lisa K. and Seim, Inge. 2021. "The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype." PEERJ. 9, pp. 1-29. https://doi.org/10.7717/peerj.10280
Article Title

The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype

ERA Journal ID211193
Article CategoryArticle
AuthorsThomas, Patrick B. (Author), Jeffrey, Penny (Author), Gahete, Manuel D. (Author), Whiteside, Eliza (Author), Walpole, Carina (Author), Maugham, Michelle (Author), Jovanovic, Lidija (Author), Gunter, Jennifer (Author), Williams, Elizabeth (Author), Nelson, Colleen (Author), Herington, Adrian (Author), Luque, Raul M. (Author), Veedu, Rakesh (Author), Chopin, Lisa K. (Author) and Seim, Inge (Author)
Journal TitlePEERJ
Journal Citation9, pp. 1-29
Article Numbere10280
Number of Pages29
Year2021
Place of PublicationUnited Kingdom
ISSN2167-8359
Digital Object Identifier (DOI)https://doi.org/10.7717/peerj.10280
Web Address (URL)https://peerj.com/articles/10280/
Abstract

It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration and survival, and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target.

Keywordslong non-coding RNA, antisense transcript, prostate cancer, tumour growth, gene expression
ANZSRC Field of Research 2020321101. Cancer cell biology
321108. Molecular targets
Byline AffiliationsQueensland University of Technology
Maimonides Institute of Biomedical Research of Cordoba, Spain
Centre for Health Research
University of Cordoba, Spain
Murdoch University
Open access urlhttps://peerj.com/articles/10280/
Institution of OriginUniversity of Southern Queensland
Funding source
NHMRC
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