The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
Article
Thomas, Patrick B., Jeffrey, Penny, Gahete, Manuel D., Whiteside, Eliza, Walpole, Carina, Maugham, Michelle, Jovanovic, Lidija, Gunter, Jennifer, Williams, Elizabeth, Nelson, Colleen, Herington, Adrian, Luque, Raul M., Veedu, Rakesh, Chopin, Lisa K. and Seim, Inge. 2021. "The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype." PEERJ. 9, pp. 1-29. https://doi.org/10.7717/peerj.10280
| Article Title | The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype |
|---|---|
| ERA Journal ID | 211193 |
| Article Category | Article |
| Authors | Thomas, Patrick B. (Author), Jeffrey, Penny (Author), Gahete, Manuel D. (Author), Whiteside, Eliza (Author), Walpole, Carina (Author), Maugham, Michelle (Author), Jovanovic, Lidija (Author), Gunter, Jennifer (Author), Williams, Elizabeth (Author), Nelson, Colleen (Author), Herington, Adrian (Author), Luque, Raul M. (Author), Veedu, Rakesh (Author), Chopin, Lisa K. (Author) and Seim, Inge (Author) |
| Journal Title | PEERJ |
| Journal Citation | 9, pp. 1-29 |
| Article Number | e10280 |
| Number of Pages | 29 |
| Year | 2021 |
| Place of Publication | United Kingdom |
| ISSN | 2167-8359 |
| Digital Object Identifier (DOI) | https://doi.org/10.7717/peerj.10280 |
| Web Address (URL) | https://peerj.com/articles/10280/ |
| Abstract | It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration and survival, and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target. |
| Keywords | long non-coding RNA, antisense transcript, prostate cancer, tumour growth, gene expression |
| ANZSRC Field of Research 2020 | 321101. Cancer cell biology |
| 321108. Molecular targets | |
| Byline Affiliations | Queensland University of Technology |
| Maimonides Institute of Biomedical Research of Cordoba, Spain | |
| Centre for Health Research | |
| University of Cordoba, Spain | |
| Murdoch University | |
| Open access url | https://peerj.com/articles/10280/ |
| Institution of Origin | University of Southern Queensland |
| Funding source | NHMRC |
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