The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

Article


Thomas, Patrick B., Seim, Inge, Jeffery, Penny L., Gahete, Manuel D., Maugham, Michelle, Crisp, Gabrielle J., Stacey, Andrew, Shah, Esha T., Walpole, Carina, Whiteside, Eliza J., Nelson, Colleen C., Herington, Adrian C., Luque, Raul M., Veedu, Rakesh N. and Chopin, Lisa K.. 2019. "The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth." International Journal of Oncology. 55 (6), pp. 1223-1236. https://doi.org/10.3892/ijo.2019.4891
Article Title

The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

ERA Journal ID16264
Article CategoryArticle
AuthorsThomas, Patrick B. (Author), Seim, Inge (Author), Jeffery, Penny L. (Author), Gahete, Manuel D. (Author), Maugham, Michelle (Author), Crisp, Gabrielle J. (Author), Stacey, Andrew (Author), Shah, Esha T. (Author), Walpole, Carina (Author), Whiteside, Eliza J. (Author), Nelson, Colleen C. (Author), Herington, Adrian C. (Author), Luque, Raul M. (Author), Veedu, Rakesh N. (Author) and Chopin, Lisa K. (Author)
Journal TitleInternational Journal of Oncology
Journal Citation55 (6), pp. 1223-1236
Number of Pages14
Year2019
Place of PublicationGreece
ISSN1019-6439
1791-2423
Digital Object Identifier (DOI)https://doi.org/10.3892/ijo.2019.4891
Web Address (URL)https://www.spandidos-publications.com/10.3892/ijo.2019.4891
Abstract

Recent evidence suggests that numerous long non-coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA-MB-231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA-MB-231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression.

Keywordslong non-coding RNA; ghrelin receptor gene; antisensetranscript; breast cancer; cell migration; tumour growth
ANZSRC Field of Research 2020321101. Cancer cell biology
321108. Molecular targets
Open access urlhttps://www.spandidos-publications.com/10.3892/ijo.2019.4891
Institution of OriginUniversity of Southern Queensland
Byline AffiliationsQueensland University of Technology
Ghrelin Research Group, Australia
Australian Prostate Cancer Research Centre, Australia
Maimonides Institute of Biomedical Research of Cordoba, Spain
Moffitt Cancer Centre, United States
University of Cordoba, Spain
Murdoch University
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