The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

Article


Thomas, Patrick B., Seim, Inge, Jeffery, Penny L., Gahete, Manuel D., Maugham, Michelle, Crisp, Gabrielle J., Stacey, Andrew, Shah, Esha T., Walpole, Carina, Whiteside, Eliza J., Nelson, Colleen C., Herington, Adrian C., Luque, Raul M., Veedu, Rakesh N. and Chopin, Lisa K.. 2019. "The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth." International Journal of Oncology. 55 (6), pp. 1223-1236. https://doi.org/10.3892/ijo.2019.4891
Article Title

The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth

ERA Journal ID16264
Article CategoryArticle
AuthorsThomas, Patrick B. (Author), Seim, Inge (Author), Jeffery, Penny L. (Author), Gahete, Manuel D. (Author), Maugham, Michelle (Author), Crisp, Gabrielle J. (Author), Stacey, Andrew (Author), Shah, Esha T. (Author), Walpole, Carina (Author), Whiteside, Eliza J. (Author), Nelson, Colleen C. (Author), Herington, Adrian C. (Author), Luque, Raul M. (Author), Veedu, Rakesh N. (Author) and Chopin, Lisa K. (Author)
Journal TitleInternational Journal of Oncology
Journal Citation55 (6), pp. 1223-1236
Number of Pages14
Year2019
Place of PublicationGreece
ISSN1019-6439
1791-2423
Digital Object Identifier (DOI)https://doi.org/10.3892/ijo.2019.4891
Web Address (URL)https://www.spandidos-publications.com/10.3892/ijo.2019.4891
Abstract

Recent evidence suggests that numerous long non-coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA-MB-231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA-MB-231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression.

Keywordslong non-coding RNA; ghrelin receptor gene; antisensetranscript; breast cancer; cell migration; tumour growth
ANZSRC Field of Research 2020321101. Cancer cell biology
321108. Molecular targets
Open access urlhttps://www.spandidos-publications.com/10.3892/ijo.2019.4891
Institution of OriginUniversity of Southern Queensland
Byline AffiliationsQueensland University of Technology
Ghrelin Research Group, Australia
Australian Prostate Cancer Research Centre, Australia
Maimonides Institute of Biomedical Research of Cordoba, Spain
Moffitt Cancer Centre, United States
University of Cordoba, Spain
Murdoch University
Permalink -

https://research.usq.edu.au/item/q57w3/the-long-non-coding-rna-ghsros-facilitates-breast-cancer-cell-migration-and-orthotopic-xenograft-tumour-growth

Download files


Published Version
Thomas et al. 2019.pdf
License: CC BY-NC-ND 4.0
File access level: Anyone

  • 152
    total views
  • 73
    total downloads
  • 3
    views this month
  • 1
    downloads this month

Export as

Related outputs

A low-cost, antimicrobial aloe-alginate hydrogel film containing Australian First Nations remedy ‘lemon myrtle oil’ (Backhousia citriodora) – Potential for incorporation into wound dressings
Seneviratne, Dinuki M., Raphael, Brooke, Whiteside, Eliza J., Windus, Louisa C.E., Kauter, Kate, Dearnaley, John D.W., Annamalai, Pratheep K., Ward, Raelene, Song, Pingan and Burey, Paulomi (Polly). 2024. " A low-cost, antimicrobial aloe-alginate hydrogel film containing Australian First Nations remedy ‘lemon myrtle oil’ (Backhousia citriodora) – Potential for incorporation into wound dressings." Heliyon. 10 (18). https://doi.org/10.1016/j.heliyon.2024.e37516
Perspectives on improving wound care for Aboriginal health workers in rural and remote communities in Queensland, Australia
King, Helena, Whiteside, Eliza, Ward, Raelene, Kauter, Kate, Byrne, Martin, Horner, Vicki, Nutter, Helen and Lea, Jackie. 2024. "Perspectives on improving wound care for Aboriginal health workers in rural and remote communities in Queensland, Australia." BMC Health Services Research. 24. https://doi.org/10.1186/s12913-024-11490-2
The advantages of microneedle patches compared to conventional needle-based drug delivery and biopsy devices in medicine
Reinke, Alissa, Whiteside, Eliza J, Windus, Louisa, Desai, Devang, Stehr, Emma and Faraji Rad, Zahra. 2024. "The advantages of microneedle patches compared to conventional needle-based drug delivery and biopsy devices in medicine." Biomedical Engineering Advances. 8. https://doi.org/10.1016/j.bea.2024.100127
Differences in total cognition and cerebrovascular function in female breast cancer survivors and cancer-free women
Downs, Tahnee L., Whiteside, Eliza J., Foot, Gaye, Mills, Dean E. and Bliss, Edward S.. 2023. "Differences in total cognition and cerebrovascular function in female breast cancer survivors and cancer-free women." The Breast. 69, pp. 358-365. https://doi.org/10.1016/j.breast.2023.03.018
Construction and Deconstruction: Materials and substances from 'waste'
Burey, Paulomi, Feldman, Jessica, Seligmann, Hannah, Song, Eric, Flynn, Matthew, Helwig, Andreas, Gharineiat, Zahra, Seneviratne, Dinuki, Whiteside, Eliza, Shelley, Tristan, Priesler, Nils, Manalo, Allan, Mirzaghorbanali, Ali, Nourizadeh, Hadi, Roberts, Michae, Nicol, Rose, Redmond, Petrea, Lynch, Mark, Dearnaley, John, ..., Germon, Geoff. 2023. "Construction and Deconstruction: Materials and substances from 'waste'." Chemistry in Australia. (June-August 2023), pp. 16-21.
Scoping review of molecular biomarkers associated with fatigue, stress, and depression in stroke survivors: A protocol
Potter, Tarynn, Gange, Nisal, Whiteside, Eliza and Gyawali, Prajwal. 2023. "Scoping review of molecular biomarkers associated with fatigue, stress, and depression in stroke survivors: A protocol." PLoS One. 18. https://doi.org/10.1371/journal.pone.0281238.
Biomarkers to measure respiratory muscle damage following inspiratory pressure threshold loading in healthy young men
Iqbal, Muneeb, Bliss, Edward, Whiteside, Eliza, Hoffman, Ben and Mills, Dean E,. 2023. "Biomarkers to measure respiratory muscle damage following inspiratory pressure threshold loading in healthy young men." Journal of Applied Physiology. 134 (4), pp. 1038-1046. https://doi.org/10.1152/japplphysiol.00656.2022
Fundamentals of Anatomy and Physiology
Chruscik, Anna, Kauter, Kate, Windus, Louisa, Whiteside, Eliza and Dooley, Leanne. 2021. Fundamentals of Anatomy and Physiology . Australia. University of Southern Queensland.
Embedding Authentic First Nations Content within Biomedical Science Curriculum
Myer, Sarah, Fossey, Uncle Wayne, Ward, Raelene and Whiteside, Eliza J.. 2022. "Embedding Authentic First Nations Content within Biomedical Science Curriculum." Australian Conference on Science and Mathematics Education (2022). Perth, Australia Sydney, Australia.
The impact of an anatomy and physiology open textbook on student satisfaction and engagement in a regional Australian university
Chruscik, Anna, Kauter, Kate, Whiteside, Anna and Windus, Louisa. 2022. "The impact of an anatomy and physiology open textbook on student satisfaction and engagement in a regional Australian university." 39th International Conference on Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education (ASCILITE 2022). Sydney, Australia 04 - 07 Dec 2022 Australia. Australasian Society for Computers in Learning in Tertiary Education (ASCILITE). https://doi.org/10.14742/APUBS.2022.183
How to do science: A guide to researching human physiology
Lexis, Louise, Julien, Brianna, Bliss, Edward, Derrington, Kate, Dooley, Leanne, Kauter, Kate, McGregor, Rowena, Myer, Sarah, Salmeron, Raquel, Suraweera, Samanthi, Whiteside, Eliza and Windus, Louisa. 2022. How to do science: A guide to researching human physiology. Australia. University of Southern Queensland.
The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
Thomas, Patrick B., Jeffrey, Penny, Gahete, Manuel D., Whiteside, Eliza, Walpole, Carina, Maugham, Michelle, Jovanovic, Lidija, Gunter, Jennifer, Williams, Elizabeth, Nelson, Colleen, Herington, Adrian, Luque, Raul M., Veedu, Rakesh, Chopin, Lisa K. and Seim, Inge. 2021. "The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype." PEERJ. 9, pp. 1-29. https://doi.org/10.7717/peerj.10280
Academic achievement and student satisfaction: moving anatomy and physiology teaching online
Chruscik, Anna, Windus, Louisa, Wang, Jianxiong, Kauter, Kate and Whiteside, Eliza. 2020. "Academic achievement and student satisfaction: moving anatomy and physiology teaching online." 2020 Australian Conference on Science and Mathematics Education (formerly UniServe Science Conference). Online 30 Sep - 02 Oct 2020 Australia.
Cells and 3D printed bioscaffolds: inter-disciplinary collaborations to engage and inspire students in biomedical science
Whiteside, Eliza, Piper, Steph and Maxwell, Andrew. 2019. "Cells and 3D printed bioscaffolds: inter-disciplinary collaborations to engage and inspire students in biomedical science." 2019 Biosciences Education Australia Network Forum (BEAN19). Melbourne, Australia 08 - 10 Dec 2019
The gut-brain axis, the human gut microbiota and their integration in the development of obesity
Bliss, Edward S. and Whiteside, Eliza. 2018. "The gut-brain axis, the human gut microbiota and their integration in the development of obesity." Frontiers in Physiology. 9, pp. 1-27. https://doi.org/10.3389/fphys.2018.00900
Dysregulated expression of the human long noncoding RNA GHSROS may influence prostate cancer progression and resistance to docetaxel
Thomas, Patrick B., Jeffrey, Penny L., Manuel, Gahete D., Whiteside, Eliza J., Maugham, Michelle, Walpole, Carina, Gunter, Jennifer H., Williams, Elizabeth D., Nelson, Colleen C., Herington, Adrian C., Luque, Raul M., Veedu, Rakesh N., Chopin, Lisa K. and Seim, Inge. 2018. "Dysregulated expression of the human long noncoding RNA GHSROS may influence prostate cancer progression and resistance to docetaxel." Annual Meeting of 2018 American Association for Cancer Research (AACR 2018). Chicago, United States 14 - 18 Apr 2018 United States. American Association for Cancer Research (AACR). https://doi.org/10.1158/1538-7445.AM2018-2452
The long non-coding RNA, GHSROS, mediates prostate cancer growth
Thomas, Patrick, Walpole, Carina Maree, Jeffery, Penny, Jovanovic, Lidija, Herington, Adrian, Nelson, Colleen, Whiteside, Eliza, Veedu, Rakesh, Seim, Inge and Chopin, Lisa. 2018. "The long non-coding RNA, GHSROS, mediates prostate cancer growth ." Annual Scientific Meeting of the Endocrine Society of Australia 2016. Gold Coast, Australia 21 - 24 Aug 2016 United Kingdom . John Wiley & Sons. https://doi.org/10.1111/cen.13259
Stress, COMT polymorphisms, and depressive symptoms in older Australian women: an exploratory study
Seib, Charrlotte, Whiteside, Eliza, Voisey, Joanne, Lee, Kathryn, Alexander, Kimberly, Humphreys, Janice, Chopin, Lisa and Anderson, Debra. 2016. "Stress, COMT polymorphisms, and depressive symptoms in older Australian women: an exploratory study." Genetic Testing and Molecular Biomarkers. 20 (8), pp. 478-481. https://doi.org/10.1089/gtmb.2015.0028
Ghrelin and cancer
Chopin, Lisa, Walpole, Carina, Seim, Inge, Cunningham, Peter, Murray, Rachael, Whiteside, Eliza, Josh, Peter and Herington, Adrian. 2011. "Ghrelin and cancer." Molecular and Cellular Endocrinology. 340 (1), pp. 65-69. https://doi.org/10.1016/j.mce.2011.04.013
Ghrelin O-acyltransferase (GOAT) is expressed in prostate cancer tissues and cell lines and expression is differentially regulated in vitro by ghrelin
Seim, Inge, Jeffery, Penny L., de Amorim, Laura, Walpole, Carina M., Fung, Jenny, Whiteside, Eliza J., Lourie, Rohan, Herington, Adrian C. and Chopin, Lisa K.. 2013. "Ghrelin O-acyltransferase (GOAT) is expressed in prostate cancer tissues and cell lines and expression is differentially regulated in vitro by ghrelin." Reproductive Biology and Endocrinology. 11 (1), pp. 70-78. https://doi.org/10.1186/1477-7827-11-70
Targeting the Long Non-Coding RNA GHSROS, a Mediator of Prostate Cancer Tumour Growth, with Antisense Oligonucleotides
Thomas, Patrick B, Walpole, Carina M, Jeffrey, Penny L, Jovanovic, Lidija, Herington, Adrian C, Nelson, Colleen C, Whiteside, Eliza, Veedu, Rakesh N, Seim, Inge and Chopin, Lisa K.. 2016. "Targeting the Long Non-Coding RNA GHSROS, a Mediator of Prostate Cancer Tumour Growth, with Antisense Oligonucleotides." 17th Asia‐Pacific Prostate Cancer Conference. Australia 03 - 31 Aug 2016 United Kingdom . John Wiley & Sons.
Location of residence and breast cancer stage: a SEER population-based analysis
Brandon, Lindsay, Youl, Pip, Baade, Peter, Whiteside, Eliza and Kimlin, Michael. 2015. "Location of residence and breast cancer stage: a SEER population-based analysis." The Breast Journal. 21 (6), pp. 693-695. https://doi.org/10.1111/tbj.12499
Stress, lifestyle, and quality of life in midlife and older Australian women: results from the stress and the health of women study
Seib, Charrlotte, Whiteside, Eliza, Lee, Kathryn, Humphreys, Janice, Dao Tran, Tiet Hanh, Chopin, Lisa and Anderson, Debra. 2014. "Stress, lifestyle, and quality of life in midlife and older Australian women: results from the stress and the health of women study." Women's Health Issues. 24 (1), pp. e43-e52. https://doi.org/10.1016/j.whi.2013.11.004
A longitudinal study of the impact of chronic psychological stress on health-related quality of life and clinical biomarkers: protocol for the Australian Healthy Aging of Women Study
Seib, Charrlotte, Whiteside, Eliza J., Humphreys, Janice, Lee, Kathryn, Thomas, Patrick, Chopin, Lisa, Crisp, Gabrielle, O'Keeffe, Angela, Kimlin, Michael, Stacey, Andrew and Anderson, Debra. 2014. "A longitudinal study of the impact of chronic psychological stress on health-related quality of life and clinical biomarkers: protocol for the Australian Healthy Aging of Women Study." BMC Public Health. 14 (1), pp. 1-8. https://doi.org/10.1186/1471-2458-14-9
You don't know what you don't know: ethics and participant consent issues for eResearch users
Ostini, Jenny, Gacenga, Francis, Pocock, Celmara and Whiteside, Eliza. 2014. "You don't know what you don't know: ethics and participant consent issues for eResearch users." eResearch Australasia 2014 Conference: Towards Unified Global Research. Melbourne, Australia 27 - 31 Oct 2014 Sydney, Australia.
Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide
Seim, Inge, Jeffrey, Penny L., Thomas, Patrick B., Walpole, Carina M., Maugham, Michelle, Fung, Jenny N. T., Yap, Pei-Yi, O'Keeffe, Angela, Lai, John, Whiteside, Eliza J., Herington, Adrian C. and Chopin, Lisa K.. 2016. "Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide." Endocrine. 52 (3), pp. 609-617. https://doi.org/10.1007/s12020-015-0848-7
Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer
Seim, Inge, Lubik, Amy A., Lehman, Melanie L., Tomlinson, Nadine, Whiteside, Eliza J., Herington, Adrian C., Nelson, Colleen C. and Chopin, Lisa K.. 2013. "Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer." Journal of Molecular Endocrinology. 50 (2), pp. 179-191. https://doi.org/10.1530/JME-12-0150
Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines
Whiteside, Eliza J., Seim, Inge, Pauli, Jana P., O'Keefe, Angela J., Thomas, Patrick B., Carter, Shea L., Walpole, Carina M., Fung, Jenny N. T., Josh, Peter, Herington, Adrian C. and Chopin, Lisa K.. 2013. "Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines." International Journal of Oncology. 43 (2), pp. 566-574. https://doi.org/10.3892/ijo.2013.1969
Isolation, structure determination and cytotoxicity studies of tryptophan alkaloids from an Australian marine sponge Hyrtios sp.
Khokhar, Shahan, Feng, Yunjiang, Campitelli, Mark R., Ekins, Merrick G., Hooper, John N. A., Beattie, Karren D., Sadowski, Martin C., Nelson, Colleen C. and Davis, Rohan A.. 2014. "Isolation, structure determination and cytotoxicity studies of tryptophan alkaloids from an Australian marine sponge Hyrtios sp." Bioorganic and Medicinal Chemistry Letters. 24 (15), pp. 3329-3332. https://doi.org/10.1016/j.bmcl.2014.05.104
Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: a role for GH in early embryogenesis?
Pantaleon, M., Whiteside, E. J., Harvey, M.B., Barnard, R.T., Waters, M.J. and Kaye, P.L.. 1997. "Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: a role for GH in early embryogenesis?" University of Adelaide. 94 (10), pp. 5125-5130. https://doi.org/10.1073/pnas.94.10.5125