The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth
Article
Thomas, Patrick B., Seim, Inge, Jeffery, Penny L., Gahete, Manuel D., Maugham, Michelle, Crisp, Gabrielle J., Stacey, Andrew, Shah, Esha T., Walpole, Carina, Whiteside, Eliza J., Nelson, Colleen C., Herington, Adrian C., Luque, Raul M., Veedu, Rakesh N. and Chopin, Lisa K.. 2019. "The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth." International Journal of Oncology. 55 (6), pp. 1223-1236. https://doi.org/10.3892/ijo.2019.4891
| Article Title | The long non-coding RNA GHSROS facilitates breast cancer cell migration and orthotopic xenograft tumour growth |
|---|---|
| ERA Journal ID | 16264 |
| Article Category | Article |
| Authors | Thomas, Patrick B. (Author), Seim, Inge (Author), Jeffery, Penny L. (Author), Gahete, Manuel D. (Author), Maugham, Michelle (Author), Crisp, Gabrielle J. (Author), Stacey, Andrew (Author), Shah, Esha T. (Author), Walpole, Carina (Author), Whiteside, Eliza J. (Author), Nelson, Colleen C. (Author), Herington, Adrian C. (Author), Luque, Raul M. (Author), Veedu, Rakesh N. (Author) and Chopin, Lisa K. (Author) |
| Journal Title | International Journal of Oncology |
| Journal Citation | 55 (6), pp. 1223-1236 |
| Number of Pages | 14 |
| Year | 2019 |
| Place of Publication | Greece |
| ISSN | 1019-6439 |
| 1791-2423 | |
| Digital Object Identifier (DOI) | https://doi.org/10.3892/ijo.2019.4891 |
| Web Address (URL) | https://www.spandidos-publications.com/10.3892/ijo.2019.4891 |
| Abstract | Recent evidence suggests that numerous long non-coding RNAs (lncRNAs) are dysregulated in cancer, and have critical roles in tumour development and progression. The present study investigated the ghrelin receptor antisense lncRNA growth hormone secretagogue receptor opposite strand (GHSROS) in breast cancer. Reverse transcription quantitative polymerase chain reaction revealed that GHSROS expression was significantly upregulated in breast tumour tissues compared with normal breast tissue. Induced overexpression of GHSROS in the MDA-MB-231 breast cancer cell line significantly increased cell migration in vitro, without affecting cell proliferation, a finding similar to our previous study on lung cancer cell lines. Microarray analysis revealed a significant repression of a small cluster of major histocompatibility class II genes and enrichment of immune response pathways; this phenomenon may allow tumour cells to better evade the immune system. Ectopic overexpression of GHSROS in the MDA-MB-231 cell line significantly increased orthotopic xenograft growth in mice, suggesting that in vitro culture does not fully capture the function of this lncRNA. This study demonstrated that GHSROS may serve a relevant role in breast cancer. Further studies are warranted to explore the function and therapeutic potential of this lncRNA in breast cancer progression. |
| Keywords | long non-coding RNA; ghrelin receptor gene; antisensetranscript; breast cancer; cell migration; tumour growth |
| ANZSRC Field of Research 2020 | 321101. Cancer cell biology |
| 321108. Molecular targets | |
| Open access url | https://www.spandidos-publications.com/10.3892/ijo.2019.4891 |
| Institution of Origin | University of Southern Queensland |
| Byline Affiliations | Queensland University of Technology |
| Ghrelin Research Group, Australia | |
| Australian Prostate Cancer Research Centre, Australia | |
| Maimonides Institute of Biomedical Research of Cordoba, Spain | |
| Moffitt Cancer Centre, United States | |
| University of Cordoba, Spain | |
| Murdoch University |
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