Targeting the Long Non-Coding RNA GHSROS, a Mediator of Prostate Cancer Tumour Growth, with Antisense Oligonucleotides

Poster


Thomas, Patrick B, Walpole, Carina M, Jeffrey, Penny L, Jovanovic, Lidija, Herington, Adrian C, Nelson, Colleen C, Whiteside, Eliza, Veedu, Rakesh N, Seim, Inge and Chopin, Lisa K.. 2016. "Targeting the Long Non-Coding RNA GHSROS, a Mediator of Prostate Cancer Tumour Growth, with Antisense Oligonucleotides." 17th Asia‐Pacific Prostate Cancer Conference. Australia 03 - 31 Aug 2016 United Kingdom . John Wiley & Sons.
Paper/Presentation Title

Targeting the Long Non-Coding RNA GHSROS, a Mediator of Prostate Cancer Tumour Growth, with Antisense Oligonucleotides

Presentation TypePoster
AuthorsThomas, Patrick B, Walpole, Carina M, Jeffrey, Penny L, Jovanovic, Lidija, Herington, Adrian C, Nelson, Colleen C, Whiteside, Eliza, Veedu, Rakesh N, Seim, Inge and Chopin, Lisa K.
Journal or Proceedings TitleBJU International
Journal Citation118 (S1), p. 25
Number of Pages1
YearAug 2016
PublisherJohn Wiley & Sons
Place of PublicationUnited Kingdom
ISSN1464-4096
1464-410X
Web Address (URL) of Paperhttps://bjui-journals.onlinelibrary.wiley.com/doi/10.1111/bju.13567
Web Address (URL) of Conference Proceedingshttps://bjui-journals.onlinelibrary.wiley.com/toc/1464410x/2016/118/S1
Conference/Event17th Asia‐Pacific Prostate Cancer Conference
Event Details
17th Asia‐Pacific Prostate Cancer Conference
Delivery
In person
Event Date
03 to end of 31 Aug 2016
Event Location
Australia
Abstract

Long non-coding RNAs (lncRNAs) play key regulatory roles in cancer progression, and are potential novel therapeutic targets. We have discovered a lncRNA which is antisense to the growth hormone secretagogue receptor (GHSR) gene, termed GHSROS. The objective of this study was to investigate the expression and function of GHSROS in prostate cancer.
GHSROS expression was investigated using quantitative RT-PCR in prostate cancer specimens. The effects of GHSROS on cell proliferation and migration were investigated using the xCELLigence system in DU145 and PC3 cell lines over-expressing GHSROS. Tumour growth was investigated in vivo using a subcutaneous xenograft model. Next generation RNA sequencing (RNAseq) was used to identify genes differentially regulated by GHSROS. Finally, the ability to silence GHSROS expression and function was demonstrated using locked nucleic acid (LNA) antisense oligonucleotide.
GHSROS, is highly expressed in a significant subset of prostate cancers. GHSROS stimulates cell proliferation and migration in prostate cancer cell lines in vitro and increases tumour xenograft growth in vivo. RNAseq demonstrated that genes associated with migration, metastasis and cell survival were significantly up-regulated by GHSROS. Using novel LNA antisense oligonucleotides we were able to demonstrate that silencing of GHSROS inhibits cell proliferation and migration in the PC3 cell line.
GHSROS may have clinical significance in prostate cancer as it is highly expressed in a significant subset of prostate cancers. GHSROS plays a role in cell proliferation, migration, and tumour growth and may provide a useful target for the development of novel antisense therapies for prostate cancer treatment.

ANZSRC Field of Research 2020321109. Predictive and prognostic markers
Public Notes

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Byline AffiliationsQueensland University of Technology
Murdoch University
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