The long non-coding RNA, GHSROS, mediates prostate cancer growth
Paper
Paper/Presentation Title | The long non-coding RNA, GHSROS, mediates prostate cancer growth |
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Presentation Type | Paper |
Authors | Thomas, Patrick, Walpole, Carina Maree, Jeffery, Penny, Jovanovic, Lidija, Herington, Adrian, Nelson, Colleen, Whiteside, Eliza, Veedu, Rakesh, Seim, Inge and Chopin, Lisa |
Journal or Proceedings Title | Clinical Endocrinology |
Journal Citation | 89 (S1), pp. 24-24 |
Number of Pages | 1 |
Year | Jun 2018 |
Publisher | John Wiley & Sons |
Place of Publication | United Kingdom |
ISSN | 0300-0664 |
1365-2265 | |
Digital Object Identifier (DOI) | https://doi.org/10.1111/cen.13259 |
Web Address (URL) of Paper | https://onlinelibrary.wiley.com/doi/10.1111/cen.13259 |
Web Address (URL) of Conference Proceedings | https://onlinelibrary.wiley.com/toc/13652265/2017/86/S1 |
Conference/Event | Annual Scientific Meeting of the Endocrine Society of Australia 2016 |
Event Details | Annual Scientific Meeting of the Endocrine Society of Australia 2016 Delivery In person Event Date 21 to end of 24 Aug 2016 Event Location Gold Coast, Australia |
Abstract | Long non-coding RNAs (lncRNAs) play key regulatory roles in cancer progression and are novel therapeutic targets1. We have discovered a lncRNA on the antisense strand of the ghrelin receptor gene (GHSR), termed GHSROS (GHSR opposite strand). Using quantitative RT-PCR we demonstrated that GHSROS is highly expressed in a subset of high grade prostate cancers. GHSROS over-expression significantly increased cell proliferation in the PC3 (1.76 ± 0.18 fold, P < 0.01) and DU145 prostate cancer cell lines compared to vector control (1.74 fold ± 0.73 P < 0.01), using xCELLigence real time cell analysis. GHSROS also increased cell migration in these cell lines compared to vector control (1.54 ± 0.35 fold in the PC3 cell line, P < 0.05, and 1.94 ± 0.43 fold in the DU145 cell line, P < 0.01). RNA sequencing in the PC3-GHSROS cell line demonstrated that genes associated with a metastatic prostate cancer gene signature were suppressed or induced by GHSROS. Using novel locked nucleic acid antisense oligonucleotides designed to target GHSROS, GHSROS silencing inhibited cell proliferation (−1.14 ± 0.07 fold, P < 0.05) and migration (−1.9 ± 0.14 fold, P < 0.05) in the PC3 cell line. Tumour growth was investigated in vivo using a subcutaneous NOD/SCID mouse xenograft model. Tumour volume was significantly increased in the PC3 and DU145 cell line xenografts over-expressing GHSROS (P < 0.05). GHSROS may have clinical significance in prostate cancer as it is highly expressed in a significant subset of prostate cancers and is associated with a metastatic gene signature. GHSROS plays a role in cell proliferation, migration and tumour growth and may provide a useful target for the development of novel antisense therapies for prostate cancer treatment. |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 321109. Predictive and prognostic markers |
321101. Cancer cell biology | |
321108. Molecular targets | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Queensland University of Technology |
Princess Alexandra Hospital, Australia | |
Centre for Health Research | |
University of Southern Queensland | |
Murdoch University |
https://research.usq.edu.au/item/yy570/the-long-non-coding-rna-ghsros-mediates-prostate-cancer-growth
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