The long non-coding RNA, GHSROS, mediates prostate cancer growth
Paper
Thomas, Patrick, Walpole, Carina Maree, Jeffery, Penny, Jovanovic, Lidija, Herington, Adrian, Nelson, Colleen, Whiteside, Eliza, Veedu, Rakesh, Seim, Inge and Chopin, Lisa. 2018. "The long non-coding RNA, GHSROS, mediates prostate cancer growth
." Annual Scientific Meeting of the Endocrine Society of Australia 2016. Gold Coast, Australia 21 - 24 Aug 2016 United Kingdom . John Wiley & Sons. https://doi.org/10.1111/cen.13259
| Paper/Presentation Title | The long non-coding RNA, GHSROS, mediates prostate cancer growth |
|---|---|
| Presentation Type | Paper |
| Authors | Thomas, Patrick, Walpole, Carina Maree, Jeffery, Penny, Jovanovic, Lidija, Herington, Adrian, Nelson, Colleen, Whiteside, Eliza, Veedu, Rakesh, Seim, Inge and Chopin, Lisa |
| Journal or Proceedings Title | Clinical Endocrinology |
| Journal Citation | 89 (S1), pp. 24-24 |
| Number of Pages | 1 |
| Year | Jun 2018 |
| Publisher | John Wiley & Sons |
| Place of Publication | United Kingdom |
| ISSN | 0300-0664 |
| 1365-2265 | |
| Digital Object Identifier (DOI) | https://doi.org/10.1111/cen.13259 |
| Web Address (URL) of Paper | https://onlinelibrary.wiley.com/doi/10.1111/cen.13259 |
| Web Address (URL) of Conference Proceedings | https://onlinelibrary.wiley.com/toc/13652265/2017/86/S1 |
| Conference/Event | Annual Scientific Meeting of the Endocrine Society of Australia 2016 |
| Event Details | Annual Scientific Meeting of the Endocrine Society of Australia 2016 Delivery In person Event Date 21 to end of 24 Aug 2016 Event Location Gold Coast, Australia |
| Abstract | Long non-coding RNAs (lncRNAs) play key regulatory roles in cancer progression and are novel therapeutic targets1. We have discovered a lncRNA on the antisense strand of the ghrelin receptor gene (GHSR), termed GHSROS (GHSR opposite strand). Using quantitative RT-PCR we demonstrated that GHSROS is highly expressed in a subset of high grade prostate cancers. GHSROS over-expression significantly increased cell proliferation in the PC3 (1.76 ± 0.18 fold, P < 0.01) and DU145 prostate cancer cell lines compared to vector control (1.74 fold ± 0.73 P < 0.01), using xCELLigence real time cell analysis. GHSROS also increased cell migration in these cell lines compared to vector control (1.54 ± 0.35 fold in the PC3 cell line, P < 0.05, and 1.94 ± 0.43 fold in the DU145 cell line, P < 0.01). RNA sequencing in the PC3-GHSROS cell line demonstrated that genes associated with a metastatic prostate cancer gene signature were suppressed or induced by GHSROS. Using novel locked nucleic acid antisense oligonucleotides designed to target GHSROS, GHSROS silencing inhibited cell proliferation (−1.14 ± 0.07 fold, P < 0.05) and migration (−1.9 ± 0.14 fold, P < 0.05) in the PC3 cell line. Tumour growth was investigated in vivo using a subcutaneous NOD/SCID mouse xenograft model. Tumour volume was significantly increased in the PC3 and DU145 cell line xenografts over-expressing GHSROS (P < 0.05). GHSROS may have clinical significance in prostate cancer as it is highly expressed in a significant subset of prostate cancers and is associated with a metastatic gene signature. GHSROS plays a role in cell proliferation, migration and tumour growth and may provide a useful target for the development of novel antisense therapies for prostate cancer treatment. |
| Contains Sensitive Content | Does not contain sensitive content |
| ANZSRC Field of Research 2020 | 321109. Predictive and prognostic markers |
| 321101. Cancer cell biology | |
| 321108. Molecular targets | |
| Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
| Byline Affiliations | Queensland University of Technology |
| Princess Alexandra Hospital, Australia | |
| Centre for Health Research | |
| University of Southern Queensland | |
| Murdoch University |
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