Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: a role for GH in early embryogenesis?

Article


Pantaleon, M., Whiteside, E. J., Harvey, M.B., Barnard, R.T., Waters, M.J. and Kaye, P.L.. 1997. "Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: a role for GH in early embryogenesis?" University of Adelaide. 94 (10), pp. 5125-5130. https://doi.org/10.1073/pnas.94.10.5125
Article Title

Functional growth hormone (GH) receptors and GH are expressed by preimplantation mouse embryos: a role for GH in early embryogenesis?

Article CategoryArticle
AuthorsPantaleon, M. (Author), Whiteside, E. J. (Author), Harvey, M.B. (Author), Barnard, R.T. (Author), Waters, M.J. (Author) and Kaye, P.L. (Author)
Journal TitleUniversity of Adelaide
Journal Citation94 (10), pp. 5125-5130
Number of Pages6
Year1997
Place of PublicationUnited States
Digital Object Identifier (DOI)https://doi.org/10.1073/pnas.94.10.5125
Web Address (URL)https://www.pnas.org/content/94/10/5125
Abstract

The results of this study challenge the widely held view that growth hormone (GH) acts only during the postnatal period. RNA phenotyping shows transcripts for the GH receptor and GH-binding protein in mouse preimplantation embryos of all stages from fertilized eggs (day 1) to blastocysts (day 4). An antibody specific to the cytoplasmic region of the GH receptor revealed receptor protein expression, first in two-cell embryos, the stage of activation of the embryonic genome (day 2), and in all subsequent stages. In cleavage-stage embryos this immunoreactivity was localized mainly to the nucleus, but clear evidence of membrane labeling was apparent in blastocysts. GH receptor immunoreactivity was also observed in cumulus cells associated with unfertilized oocytes but not in the unfertilized oocytes. The blastocyst receptor was demonstrated to be functional, exhibiting the classic bell-shaped dose–response curves for GH stimulation of both 3-O-methyl glucose transport and protein synthesis. Maximal stimulation of 40–50% was seen for both responses at less than 1 ng/ml recombinant GH, suggesting a role for maternal GH. However mRNA transcripts for GH were also detected from the morula stage (day 3) by using reverse transcription–PCR, and GH immunoreactivity was seen in blastocysts. These observations raise the possibility of a paracrine/autocrine GH loop regulating embryonic development in its earliest stages.

KeywordsPreimplantation physiology, protein synthesis, glucose transport
ANZSRC Field of Research 2020321503. Reproduction
Institution of OriginUniversity of Southern Queensland
Byline AffiliationsUniversity of Queensland
Queensland University of Technology
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