Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines

Article


Whiteside, Eliza J., Seim, Inge, Pauli, Jana P., O'Keefe, Angela J., Thomas, Patrick B., Carter, Shea L., Walpole, Carina M., Fung, Jenny N. T., Josh, Peter, Herington, Adrian C. and Chopin, Lisa K.. 2013. "Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines." International Journal of Oncology. 43 (2), pp. 566-574. https://doi.org/10.3892/ijo.2013.1969
Article Title

Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines

ERA Journal ID16264
Article CategoryArticle
AuthorsWhiteside, Eliza J. (Author), Seim, Inge (Author), Pauli, Jana P. (Author), O'Keefe, Angela J. (Author), Thomas, Patrick B. (Author), Carter, Shea L. (Author), Walpole, Carina M. (Author), Fung, Jenny N. T. (Author), Josh, Peter (Author), Herington, Adrian C. (Author) and Chopin, Lisa K. (Author)
Journal TitleInternational Journal of Oncology
Journal Citation43 (2), pp. 566-574
Number of Pages9
Year2013
Place of PublicationAthens, Greece
ISSN1019-6439
1791-2423
Digital Object Identifier (DOI)https://doi.org/10.3892/ijo.2013.1969
Web Address (URL)http://www.spandidos-publications.com/10.3892/ijo.2013.1969
Abstract

The molecular mechanisms involved in non-small cell lung cancer tumourigenesis are largely unknown; however, recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a role. In this study, we used public databases to identify an mRNA-like, candidate long non-coding RNA, GHSROS (GHSR opposite strand), transcribed from the antisense strand of the ghrelin receptor gene, growth hormone secretagogue receptor (GHSR). Quantitative real-time RT-PCR revealed higher expression of GHSROS in lung cancer tissue compared to adjacent, non-tumour lung tissue. In common with many long non-coding RNAs, GHSROS is 5′ capped and 3′ polyadenylated (mRNA-like), lacks an extensive open reading frame and harbours a transposable element. Engineered overexpression of GHSROS stimulated cell migration in the A549 and NCI-H1299 non-small cell lung cancer cell lines, but suppressed cell migration in the Beas-2B normal lung-derived bronchoepithelial cell line. This suggests that GHSROS function may be dependent on the oncogenic context. The identification of GHSROS, which is expressed in lung cancer and stimulates cell migration in lung cancer cell lines, contributes to the growing number of non-coding RNAs that play a role in the regulation of tumourigenesis and metastatic cancer progression.

Keywordsantisense transcript; cell migration; ghrelin receptor gene; long non-coding RNA; lung cancer; non-small cell lung carcinoma
ANZSRC Field of Research 2020321101. Cancer cell biology
321108. Molecular targets
310506. Gene mapping
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Byline AffiliationsQueensland University of Technology
University of Queensland
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
Institution of OriginUniversity of Southern Queensland
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