Enhancing the value of nitrogen from rapeseed meal for microbial oil production
Article
Article Title | Enhancing the value of nitrogen from rapeseed meal for microbial oil production |
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ERA Journal ID | 3358 |
Article Category | Article |
Authors | Kiran, Esra Uckun (Author), Salakkama, Apilak (Author), Trzcinski, Antoine P. (Author), Bakir, Ufuk (Author) and Webb, Colin (Author) |
Journal Title | Enzyme and Microbial Technology |
Journal Citation | 50 (6-7), pp. 337-342 |
Number of Pages | 6 |
Year | 2012 |
Place of Publication | United States |
ISSN | 0141-0229 |
1879-0909 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.enzmictec.2012.03.004 |
Web Address (URL) | http://www.sciencedirect.com/science/article/pii/S014102291200035X |
Abstract | Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g−1 dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g−1), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g−1), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g−1), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g−1). The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3 g dry cell L−1 of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%. The dry cell weight obtained was higher than that obtained using conventional yeast extract, due to a higher total nitrogen content in the novel biomedium. The fatty acids obtained from the microbial oil were similar to those derived from rapeseed oil. |
Keywords | rapeseed meal; fermentation; solid state fermentation; free amino nitrogen; Rhodosporidium toruloides; microbial oil |
ANZSRC Field of Research 2020 | 410404. Environmental management |
401102. Environmentally sustainable engineering | |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Byline Affiliations | University of Manchester, United Kingdom |
Middle East Technical University, Turkey | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q3644/enhancing-the-value-of-nitrogen-from-rapeseed-meal-for-microbial-oil-production
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