Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness

Article


Denham, Joshua and Prestes, Priscilla R.. 2016. "Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness." Frontiers in Genetics. 7, pp. 1-8. https://doi.org/10.3389/fgene.2016.00196
Article Title

Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness

ERA Journal ID210481
Article CategoryArticle
AuthorsDenham, Joshua (Author) and Prestes, Priscilla R. (Author)
Journal TitleFrontiers in Genetics
Journal Citation7, pp. 1-8
Article Number196
Number of Pages8
Year2016
Place of PublicationSwitzerland
ISSN1664-8021
Digital Object Identifier (DOI)https://doi.org/10.3389/fgene.2016.00196
Web Address (URL)https://www.frontiersin.org/articles/10.3389/fgene.2016.00196/full
Abstract

MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally. Evidence indicating miRNAs influence exercise-induced health and performance adaptations is mounting. Circulating miRNAs are responsible for intercellular communication and could serve as biomarkers for disease and exercise-related traits. Such biomarkers would contribute to exercise screening, monitoring, and the development of personalized exercise prescription. Accordingly, we investigated the impact of long-term strenuous aerobic exercise training and a single bout of maximal aerobic exercise on five muscle-enriched miRNAs implicated in exercise adaptations (miR-1, miR-133a, miR-181a, miR-486, and miR-494). We also determined linear correlations between miRNAs, resting heart rate, and maximum oxygen uptake (V˙O2 max). We used TaqMan assay quantitative polymerase chain reaction to analyze the abundance of miR-1, miR-133a, miR-181a, miR-486, and miR-494 in resting whole blood of 67 endurance athletes and 61 healthy controls. Relative to controls, endurance athletes exhibited increased miR-1, miR-486, and miR-494 content (1.26- to 1.58-fold change, all p < 0.05). miR-1, miR-133a, and miR-486 were decreased immediately after maximal aerobic exercise (0.64- to 0.76-fold change, all p < 0.01) performed by 19 healthy, young men (20.7 ± 2.4 years). Finally, we observed positive correlations between miRNA abundance and V˙ O2 max (miR-1 and miR-486) and an inverse correlation between miR-486 and resting heart rate. Therefore, muscle-enriched miRNAs isolated from whole blood are regulated by acute and long-term aerobic exercise training and could serve as biomarkers of cardiorespiratory fitness.

KeywordsEpigenetics; MyomiR; Non-coding RNA; Small RNA; VO2 max
ANZSRC Field of Research 2020310599. Genetics not elsewhere classified
420702. Exercise physiology
Public Notes

Copyright © 2016 Denham and Prestes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or
reproduction is permitted which does not comply with these terms.

Byline AffiliationsUniversity of New England
Federation University
Institution of OriginUniversity of Southern Queensland
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