Co-expression analysis identifies networks of miRNAs implicated in biological ageing and modulated by short-term interval training

Article


Dev, Prasun Kumar, Gray, Adrian J., Scott-Hamilton, John, Hagstrom, Amanda D., Murphy, Aron and Denham, Joshua. 2021. "Co-expression analysis identifies networks of miRNAs implicated in biological ageing and modulated by short-term interval training." Mechanisms of Ageing and Development. 199. https://doi.org/10.1016/j.mad.2021.111552
Article Title

Co-expression analysis identifies networks of miRNAs implicated in biological ageing and modulated by short-term interval training

ERA Journal ID16583
Article CategoryArticle
AuthorsDev, Prasun Kumar (Author), Gray, Adrian J. (Author), Scott-Hamilton, John (Author), Hagstrom, Amanda D. (Author), Murphy, Aron (Author) and Denham, Joshua (Author)
Journal TitleMechanisms of Ageing and Development
Journal Citation199
Article Number111552
Number of Pages13
Year2021
Place of PublicationIreland
ISSN0047-6374
1872-6216
Digital Object Identifier (DOI)https://doi.org/10.1016/j.mad.2021.111552
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S004763742100124X?via%3Dihub
Abstract

Exercise training seems to promote healthy biological ageing partly by inducing telomere maintenance, yet the molecular mechanisms are not fully understood. Recent studies have emphasised the importance of microRNAs (miRNAs) in ageing and their ability to mirror pathophysiological alterations associated with age-related dis- eases. We examined the association between aerobic fitness and leukocyte telomere length before determining the influence of vigorous exercise training on the regulation of leukocyte miRNA networks. Telomere length was positively correlated to aerobic fitness (r = 0.32, p = 0.02). 104 miRNAs were differentially expressed after six weeks of thrice-weekly sprint interval training (SIT) in healthy men (q < 0.05). Gene co-expression analysis (WGCNA) detected biologically meaningful miRNA networks, five of which were significantly correlated with pre-SIT and post-SIT expression profiles (p < 0.001) and telomere length. Enrichment analysis revealed that the immune response, T cell differentiation and lipid metabolism associated miRNAs clusters were significantly down-regulated after SIT. Using data acquired from the Gene Expression Omnibus (GEO), we also identified two co-expressed miRNAs families that were modulated by exercise training in previous investigations. Collectively, our findings highlight the miRNA networks implicated in exercise adaptations and telomere regulation, and suggest that SIT may attenuate biological ageing through the control of the let-7 and miR-320 miRNA families.

Keywordssmall non-coding RNA, exercise training, telomere length, gene, WGCNA
ANZSRC Field of Research 2020420799. Sports science and exercise not elsewhere classified
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Byline AffiliationsCentral University of South Bihar, India
University of New England
University of New South Wales
Royal Melbourne Institute of Technology (RMIT)
Institution of OriginUniversity of Southern Queensland
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