Inspiratory flow-resistive breathing, respiratory muscle-induced systemic oxidative stress, and diaphragm fatigue in healthy humans

Article

Briskey, David R., Vogel, Kurt, Johnson, Michael A., Sharpe, Graham R., Coombes, Jeff S. and Mills, Dean E.. 2020. "Inspiratory flow-resistive breathing, respiratory muscle-induced systemic oxidative stress, and diaphragm fatigue in healthy humans." Journal of Applied Physiology. 129 (1), pp. 185-193. https://doi.org/10.1152/japplphysiol.00091.2020
Article Title

Inspiratory flow-resistive breathing, respiratory muscle-induced systemic oxidative stress, and diaphragm fatigue in healthy humans

ERA Journal ID3161
Article CategoryArticle
AuthorsBriskey, David R. (Author), Vogel, Kurt (Author), Johnson, Michael A. (Author), Sharpe, Graham R. (Author), Coombes, Jeff S. (Author) and Mills, Dean E. (Author)
Journal TitleJournal of Applied Physiology
Journal Citation129 (1), pp. 185-193
Number of Pages9
Year2020
PublisherAmerican Physiological Society
Place of PublicationUnited States
ISSN1522-1601
8750-7587
Digital Object Identifier (DOI)https://doi.org/10.1152/japplphysiol.00091.2020
Web Address (URL)https://journals.physiology.org/doi/abs/10.1152/japplphysiol.00091.2020
Abstract

We questioned whether the respiratory muscles of humans contribute to systemic oxidative stress following inspiratory flow-resistive breathing, whether the amount of oxidative stress is influenced by the level of resistive load, and whether the amount of oxidative stress is related to the degree of diaphragm fatigue incurred. Eight young and healthy participants attended the laboratory for four visits on separate days. During the first visit, height, body mass, lung function, and maximal inspiratory mouth and transdiaphragmatic pressure (Pdimax) were assessed. During visits 2–4, participants undertook inspiratory flow-resistive breathing with either no resistance (control) or resistive loads equivalent to 50 and 70% of their Pdimax (Pdimax50% and Pdimax70%) for 30 min. Participants undertook one resistive load per visit, and the order in which they undertook the loads was randomized. Inspiratory muscle pressures were higher (P < 0.05) during the 5th and Final min of Pdimax50% and Pdimax70% compared with control. Plasma F2-isoprostanes increased (P < 0.05) following inspiratory flow-resistive breathing at Pdimax70%. There were no increases in plasma protein carbonyls or total antioxidant capacity. Furthermore, although we evidenced small reductions in transdiapragmaic twitch pressures (PdiTW) after inspiratory flow-resistive breathing at Pdimax50% and Pdimax70%, this was not related to the increase in plasma F2-isoprostanes. Our novel data suggest that it is only when sufficiently strenuous that inspiratory flow-resistive breathing in humans elicits systemic oxidative stress evidenced by elevated plasma F2-isoprostanes, and based on our data, this is not related to a reduction in PdiTW.

Keywordsdiaphragm fatigue; humans; inspiratory flow resistive breathing; oxidative stress; respiratory muscles
ANZSRC Field of Research 2020420702. Exercise physiology
320103. Respiratory diseases
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Byline AffiliationsUniversity of Queensland
Centre for Health Research
Nottingham Trent University, United Kingdom
Centre for Health, Informatics and Economic Research
Institution of OriginUniversity of Southern Queensland
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