Antioxidant supplementation enhances erythrocyte antioxidant status and attenuates cyclosporine-induced vascular dysfunction
Article
Article Title | Antioxidant supplementation enhances erythrocyte antioxidant status and attenuates cyclosporine-induced vascular dysfunction |
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ERA Journal ID | 15663 |
Article Category | Article |
Authors | Lexis, L. A. (Author), Fenning, A. (Author), Brown, L. (Author), Fassett, R. G. (Author) and Coombes, J. S. (Author) |
Journal Title | American Journal of Transplantation |
Journal Citation | 6 (1), pp. 41-49 |
Number of Pages | 9 |
Year | 2006 |
ISSN | 1600-6135 |
1600-6143 | |
Digital Object Identifier (DOI) | https://doi.org/10.1111/j.1600-6143.2005.01154.x |
Abstract | The aim of this study was to determine the effects of dietary antioxidant supplementation with alpha-tocopherol and alpha-lipoic acid on cyclosporine-induced alterations to erythrocyte and plasma redox balance, and cyclosporine-induced endothelial and smooth muscle dysfunction. Rats were randomly assigned to either control, antioxidant, cyclosporine or cyclosporine + antioxidant treatments. Cyclosporine A was administered for 10 days after an 8-week feeding period. Plasma was analyzed for alpha-tocopherol, total antioxidant capacity, malondialdehyde and creatinine. Erythrocytes were analyzed for glutathione, methemoglobin, superoxide dismutase, catalase, glutathione peroxidase, glucose-6-phosphate dehydrogenase, alpha-tocopherol and malondialdehye. Vascular endothelial and smooth muscle function was determined in vitro. Antioxidant supplementation resulted in significant increases in erythrocyte alpha-tocopherol concentration and glutathione peroxidase activity in both of the antioxidant-supplemented groups. Cyclosporine administration caused significant decreases in glutathione concentration, methemoglobin concentration and superoxide dismutase activity. Antioxidant supplementation attenuated the cyclosporine-induced decrease in superoxide dismutase activity. Cyclosporine therapy impaired both endothelium-independent and -dependent relaxation of the thoracic aorta, and this was attenuated by antioxidant supplementation. In summary, dietary supplementation with alpha-tocopherol and alpha-lipoic acid attenuated the cyclosporine-induced decrease in erythrocyte superoxide dismutase activity and attenuated cyclosporine-induced vascular dysfunction. |
Keywords | antioxidant status, cyclosporine A, erythrocytes, oxidative stress, vascular function |
ANZSRC Field of Research 2020 | 319999. Other biological sciences not elsewhere classified |
310999. Zoology not elsewhere classified | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | La Trobe University |
University of Queensland | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q0yq3/antioxidant-supplementation-enhances-erythrocyte-antioxidant-status-and-attenuates-cyclosporine-induced-vascular-dysfunction
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