Inhibition of inflammation and fibrosis by a complement C5a receptor antagonist in DOCA-salt hypertensive rats
Article
Article Title | Inhibition of inflammation and fibrosis by a complement C5a receptor antagonist in DOCA-salt hypertensive rats |
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ERA Journal ID | 14789 |
Article Category | Article |
Authors | Iyer, Abishek (Author), Woodruff, Trent M. (Author), Wu, Mike C. L. (Author), Stylianou, Con (Author), Reid, Robert C. (Author), Fairlie, David P. (Author), Taylor, Stephen M. (Author) and Brown, Lindsay (Author) |
Journal Title | Journal of Cardiovascular Pharmacology |
Journal Citation | 58 (5), pp. 479-486 |
Number of Pages | 8 |
Year | 2011 |
Place of Publication | United States |
ISSN | 0160-2446 |
1533-4023 | |
Digital Object Identifier (DOI) | https://doi.org/10.1097/FJC.0b013e31822a7a09 |
Web Address (URL) | http://journals.lww.com/cardiovascularpharm/pages/articleviewer.aspx?year=2011&issue=11000&article=00005&type=abstract |
Abstract | The anaphylatoxin C5a generated by activation of the innate immunity complement system is a potent inflammatory peptide mediator through the G-protein-coupled receptor C5aR (CD88) present in immune-inflammatory cells, including monocytes, macrophages, neutrophils, T cells, and mast cells. Inflammatory cells infiltrate and initiate the development of fibrosis in the chronically hypertensive heart. In this study, we have investigated whether treatment with a selective C5aR antagonist prevents Inhibition of inflammation and fibrosis by a complement C5a receptor antagonist in DOCA-salt hypertensive rats in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Control and DOCA-salt rats were treated with PMX53 (AcF-[OPdChaWR], 1 mg·kg·d oral gavage) for 32 days; structural and functional changes in cardiovascular system were determined. DOCA-salt hypertension increased leukocyte extravasation into ventricular tissue, increasing collagen deposition and ventricular stiffness; PMX53 treatment attenuated these changes, thereby improving cardiac function. Further, treatment with PMX53 suppressed an increased expression of C5aR in the left ventricle from DOCA-salt rats, consistent with the reduced infiltration of inflammatory cells. Vascular endothelial dysfunction in thoracic aortic rings was attenuated by PMX53 treatment, but systolic blood pressure was unchanged in DOCA-salt rats. In the heart, PMX53 treatment attenuated inflammatory cell infiltration, fibrosis, and ventricular stiffness, indicating that C5aR is critically involved in ventricular remodeling by regulating inflammatory responses in the hypertensive heart. |
Keywords | hypertension; inflammation; fibrosis; receptor antagonist |
ANZSRC Field of Research 2020 | 320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies) |
310112. Structural biology (incl. macromolecular modelling) | |
320101. Cardiology (incl. cardiovascular diseases) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | University of Queensland |
Department of Biological and Physical Sciences | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q12q2/inhibition-of-inflammation-and-fibrosis-by-a-complement-c5a-receptor-antagonist-in-doca-salt-hypertensive-rats
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