Fish oil in comparison to folinic acid for protection against adverse effects of methotrexate chemotherapy on bone
Article
Article Title | Fish oil in comparison to folinic acid for protection against adverse effects of methotrexate chemotherapy on bone |
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ERA Journal ID | 16462 |
Article Category | Article |
Authors | Raghu Nadhanan, Rethi (Author), Fan, Chia Ming (Author), Su, Yu Wen (Author), Howe, Peter R. C. (Author) and Xian, Cory J. (Author) |
Journal Title | Journal of Orthopaedic Research |
Journal Citation | 32 (4), pp. 587-596 |
Number of Pages | 10 |
Year | 2014 |
Place of Publication | United States |
ISSN | 0736-0266 |
1554-527X | |
Digital Object Identifier (DOI) | https://doi.org/10.1002/jor.22565 |
Web Address (URL) | http://onlinelibrary.wiley.com/doi/10.1002/jor.22565/epdf |
Abstract | Methotrexate (MTX) chemotherapy is known to cause bone loss which lacks specific preventative treatments, although clinically folinic acid is often used to reduce MTX toxicity in soft tissues. This study investigated damaging effects of MTX injections (0.75 mg/kg/day for 5 days) in rats and potential protective benefits of fish oil (0.25, 0.5, or 0.75 ml/100 g/day) in comparison to folinic acid (0.75 mg/kg) in the tibial metaphysis. MTX treatment significantly reduced height of primary spongiosa and volume of trabecular bone while reducing density of osteoblasts. Consistently, MTX reduced osteogenic differentiation but increased adipogenesis of bone marrow stromal cells, accompanied by lower mRNA expression of osteogenic transcription factors Runx2 and Osx, but an up-regulation of adipogenesis-related genes FABP4 and PPAR-γ. MTX also increased osteoclast density, bone marrow osteoclast formation, and mRNA expression of proinflammatory cytokines IL-1, IL-6, TNF-α, and RANKL/OPG ratio in bone. Fish oil (0.5 or 0.75 ml/100 g) or folinic acid supplementation preserved bone volume, osteoblast density, and osteogenic differentiation, and suppressed MTX-induced cytokine expression, osteoclastogenesis, and adipogenesis. Thus, fish oil at 0.5 ml/100 g or above is as effective as folinic acid in counteracting MTX-induced bone damage, conserving bone formation, suppressing resorption and marrow adiposity, suggesting its therapeutic potential in preventing bone loss during MTX chemotherapy. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:587-596, 2014. |
Keywords | cancer chemotherapy; bone loss; bone marrow adiposity; fish oil; folinic acid |
ANZSRC Field of Research 2020 | 321105. Chemotherapy |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | University of South Australia |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q40z5/fish-oil-in-comparison-to-folinic-acid-for-protection-against-adverse-effects-of-methotrexate-chemotherapy-on-bone
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